Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Nov;8(11):4625-33.
doi: 10.1128/mcb.8.11.4625-4633.1988.

Posttranscriptional regulation of cytochrome c expression during the developmental cycle of Trypanosoma brucei

Affiliations

Posttranscriptional regulation of cytochrome c expression during the developmental cycle of Trypanosoma brucei

A F Torri et al. Mol Cell Biol. 1988 Nov.

Abstract

We examined the expression of a nucleus-encoded mitochondrial protein, cytochrome c, during the life cycle of Trypanosoma brucei. The bloodstream forms of T. brucei, the long slender and short stumpy trypanosomes, have inactive mitochondria with no detectable cytochrome-mediated respiration. The insect form of T. brucei, the procyclic trypanosomes, has fully functional mitochondria. Cytochrome c is spectrally undetectable in the bloodstream forms of trypanosomes, but during differentiation to the procyclic form, spectrally detected holo-cytochrome c accumulates rapidly. We have purified T. brucei cytochrome c and raised antibodies that react to both holo- and apo-cytochrome c. In addition, we isolated a partial cDNA to trypanosome cytochrome c. An examination of protein expression and steady-state mRNA levels in T. brucei indicated that bloodstream trypanosomes did not express cytochrome c but maintained significant steady-state levels of cytochrome c mRNA. The results suggest that in T. brucei, cytochrome c is developmentally regulated by a posttranscriptional mechanism which prevents either translation or accumulation of cytochrome c in the bloodstream trypanosomes.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Biol Chem. 1966 Jan 10;241(1):251-3 - PubMed
    1. Mol Cell Biol. 1988 Apr;8(4):1591-601 - PubMed
    1. Nature. 1967 Jun 24;214(5095):1302-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed

Publication types

MeSH terms

Associated data

LinkOut - more resources