The effect of histone gene deletions on chromatin structure in Saccharomyces cerevisiae
- PMID: 2847314
- DOI: 10.1126/science.2847314
The effect of histone gene deletions on chromatin structure in Saccharomyces cerevisiae
Abstract
As a way of studying nucleosome assembly and maintenance in Saccharomyces cerevisiae, mutants bearing deletions or duplications of the genes encoding histones H2A and H2B were analyzed. Previous genetic analysis had shown that only one of these mutants exhibited dramatic and pleiotropic phenotypes. This mutant was also the only one that contained disrupted chromatin, suggesting that the original phenotypes were attributable to alterations in chromosome structure. The chromatin disruption in the mutant, however, did not extend over the entire genome, but rather was localized to specific regions. Thus, while the arrangement of nucleosomes over the HIS4 and GAL1 genes, the telomeres, and the long terminal repeats (delta sequences) of Ty retrotransposons appeared essentially normal, nucleosomes over the CYH2 and UBI4 genes and the centromere of chromosome III were dramatically disrupted. The observation that the mutant exhibited localized chromatin disruptions implies that the assembly or maintenance of nucleosomes differs over different parts of the yeast genome.
Similar articles
-
Nucleosome alterations caused by mutations at modifiable histone residues in Saccharomyces cerevisiae.Sci Rep. 2015 Oct 26;5:15583. doi: 10.1038/srep15583. Sci Rep. 2015. PMID: 26498326 Free PMC article.
-
Changes in histone gene dosage alter transcription in yeast.Genes Dev. 1988 Feb;2(2):150-9. doi: 10.1101/gad.2.2.150. Genes Dev. 1988. PMID: 2834270
-
Histone H2A is required for normal centromere function in Saccharomyces cerevisiae.EMBO J. 2000 Apr 3;19(7):1598-612. doi: 10.1093/emboj/19.7.1598. EMBO J. 2000. PMID: 10747028 Free PMC article.
-
Histones, nucleosomes and transcription.Curr Opin Genet Dev. 1993 Apr;3(2):219-25. doi: 10.1016/0959-437x(93)90026-l. Curr Opin Genet Dev. 1993. PMID: 8389216 Review.
-
Chromatin structure and transcription.Annu Rev Cell Biol. 1992;8:563-87. doi: 10.1146/annurev.cb.08.110192.003023. Annu Rev Cell Biol. 1992. PMID: 1335747 Review. No abstract available.
Cited by
-
Progression into the first meiotic division is sensitive to histone H2A-H2B dimer concentration in Saccharomyces cerevisiae.Genetics. 1997 Mar;145(3):647-59. doi: 10.1093/genetics/145.3.647. Genetics. 1997. PMID: 9055075 Free PMC article.
-
Global alterations in chromatin accessibility associated with loss of SIN4 function.Nucleic Acids Res. 1997 Mar 15;25(6):1240-7. doi: 10.1093/nar/25.6.1240. Nucleic Acids Res. 1997. PMID: 9092635 Free PMC article.
-
Histone regulatory (hir) mutations suppress delta insertion alleles in Saccharomyces cerevisiae.Genetics. 1991 Aug;128(4):729-38. doi: 10.1093/genetics/128.4.729. Genetics. 1991. PMID: 1655565 Free PMC article.
-
Distinct roles for S. cerevisiae H2A copies in recombination and repeat stability, with a role for H2A.1 threonine 126.Elife. 2019 Dec 5;8:e53362. doi: 10.7554/eLife.53362. Elife. 2019. PMID: 31804179 Free PMC article.
-
SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae.Mol Cell Biol. 1994 Aug;14(8):5223-8. doi: 10.1128/mcb.14.8.5223-5228.1994. Mol Cell Biol. 1994. PMID: 8035801 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
