Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jun:506:141-151.
doi: 10.1016/j.virol.2017.03.012. Epub 2017 Apr 6.

The MVMp P4 promoter is a host cell-type range determinant in vivo

Chen Meir  1 , Michal Mincberg  1 , Irina Rostovsky  1 , Saar Tal  1 , Ellen M Vollmers  2 , Adi Levi  1 , Peter Tattersall  2 , Claytus Davis  3
Affiliations
Free article

The MVMp P4 promoter is a host cell-type range determinant in vivo

Chen Meir et al. Virology. 2017 Jun.
Free article

Abstract

The protoparvovirus early promoters, e.g. P4 of Minute Virus of Mice (MVM), play a critical role during infection. Initial P4 activity depends on the host transcription machinery only. Since this is cell-type dependent, it is hypothesized that P4 is a host cell-type range determinant. Yet host range determinants have mapped mostly to capsid, never P4. Here we test the hypothesis using the mouse embryo as a model system. Disruption of the CRE element of P4 drastically decreased infection levels without altering range. However, when we swapped promoter elements of MVM P4 with those from equivalent regions of the closely related H1 virus, we observed elimination of infection in fibroblasts and chondrocytes and the acquisition of infection in skeletal muscle. We conclude that P4 is a host range determinant and a target for modifying the productive infection potential of the virus - an important consideration in adapting these viruses for oncotherapy.

Keywords: Autonomous parvovirus; Host-range determinant; MVM; P4 promoter; Protoparvovirus; Viral transcription.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

Publication types

MeSH terms

Substances

LinkOut - more resources