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. 1988 Jun;85(12):4237-41.
doi: 10.1073/pnas.85.12.4237.

Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene

C Koch  1 , J Vandekerckhove, R Kahmann
Affiliations

Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene

C Koch et al. Proc Natl Acad Sci U S A. 1988 Jun.

Abstract

The Escherichia coli (Es. coli) protein Fis (factor for inversion stimulation) stimulates site-specific DNA inversion of the G segment in phage Mu by binding to a recombinational enhancer. By using synthetic oligonucleotides deduced from the amino-terminal amino acid sequence, we have cloned the gene (termed fis) encoding this specific DNA-binding protein. The DNA sequence shows that the Fis protein is basic and contains 98 amino acids. A helix-turn-helix sequence motif characteristic of many DNA-binding proteins is located at the carboxyl-terminal end of the protein. By marker exchange, we have constructed an insertion mutation of fis. Fis is nonessential for Es. coli growth; however, inversion of the G segment of a Mu prophage was not detected in the fis mutant. The fis gene is located between 71 and 72 min on the Es. coli genetic map.

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