Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

doi:10.3791/55300

. 2017 Mar 9:(121):55300.

doi: 10.3791/55300.

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Keith C Heyde¹, Felicia Y Scott², Sung-Ho Paek², Ruihua Zhang², Warren C Ruder³

Affiliations

¹ Department of Mechanical Engineering, Carnegie Mellon University; Engineering Science and Mechanics Program, Virginia Polytechnic Institute and State University.
² Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University.
³ Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University; Department of Bioengineering, University of Pittsburgh; warrenr@pitt.edu.

PMID: 28362372
PMCID: PMC5409335
DOI: 10.3791/55300

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Keith C Heyde et al. J Vis Exp. 2017.

. 2017 Mar 9:(121):55300.

doi: 10.3791/55300.

Authors

Keith C Heyde¹, Felicia Y Scott², Sung-Ho Paek², Ruihua Zhang², Warren C Ruder³

Affiliations

¹ Department of Mechanical Engineering, Carnegie Mellon University; Engineering Science and Mechanics Program, Virginia Polytechnic Institute and State University.
² Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University.
³ Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University; Department of Bioengineering, University of Pittsburgh; warrenr@pitt.edu.

PMID: 28362372
PMCID: PMC5409335
DOI: 10.3791/55300

Abstract

We have developed an abiotic-biotic interface that allows engineered cells to control the material properties of a functionalized surface. This system is made by creating two modules: a synthetically engineered strain of E. coli cells and a functionalized material interface. Within this paper, we detail a protocol for genetically engineering selected behaviors within a strain of E. coli using molecular cloning strategies. Once developed, this strain produces elevated levels of biotin when exposed to a chemical inducer. Additionally, we detail protocols for creating two different functionalized surfaces, each of which is able to respond to cell-synthesized biotin. Taken together, we present a methodology for creating a linked, abiotic-biotic system that allows engineered cells to control material composition and assembly on nonliving substrates.

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References

1. Zhang R, Heyde KC, Scott FY, Paek S-H, Ruder WC. Programming Surface Chemistry with Engineered Cells. ACS Synth. Biol. 2016. - PubMed
1. Zhou X, et al. Reduced graphene oxide films used as matrix of MALDI-TOF-MS for detection of octachlorodibenzo-p-dioxin. Chem. Commun. 2010;46:6974–6976. - PubMed
1. Pardee K, et al. Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016;165:1255–1266. - PubMed
1. Bähring S, et al. Design and Sensing Properties of a Self-Assembled Supramolecular Oligomer. Chem. Eur. J. 2016;22:1958–1967. - PubMed
1. Nicolau Jr DV, Armitage JP, Maini PK. Directional persistence and the optimality of run-and-tumble chemotaxis. Comp. Biol. Chem. 2009;33:269–274. - PubMed

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[1] Zhang R, Heyde KC, Scott FY, Paek S-H, Ruder WC. Programming Surface Chemistry with Engineered Cells. ACS Synth. Biol. 2016. - PubMed

[2] Zhang R, Heyde KC, Scott FY, Paek S-H, Ruder WC. Programming Surface Chemistry with Engineered Cells. ACS Synth. Biol. 2016. - PubMed

[3] Zhou X, et al. Reduced graphene oxide films used as matrix of MALDI-TOF-MS for detection of octachlorodibenzo-p-dioxin. Chem. Commun. 2010;46:6974–6976. - PubMed

[4] Zhou X, et al. Reduced graphene oxide films used as matrix of MALDI-TOF-MS for detection of octachlorodibenzo-p-dioxin. Chem. Commun. 2010;46:6974–6976. - PubMed

[5] Pardee K, et al. Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016;165:1255–1266. - PubMed

[6] Pardee K, et al. Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. Cell. 2016;165:1255–1266. - PubMed

[7] Bähring S, et al. Design and Sensing Properties of a Self-Assembled Supramolecular Oligomer. Chem. Eur. J. 2016;22:1958–1967. - PubMed

[8] Bähring S, et al. Design and Sensing Properties of a Self-Assembled Supramolecular Oligomer. Chem. Eur. J. 2016;22:1958–1967. - PubMed

[9] Nicolau Jr DV, Armitage JP, Maini PK. Directional persistence and the optimality of run-and-tumble chemotaxis. Comp. Biol. Chem. 2009;33:269–274. - PubMed

[10] Nicolau Jr DV, Armitage JP, Maini PK. Directional persistence and the optimality of run-and-tumble chemotaxis. Comp. Biol. Chem. 2009;33:269–274. - PubMed

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Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

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Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

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