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. 1988 May;85(10):3294-8.
doi: 10.1073/pnas.85.10.3294.

Cloning and expression of full-length cDNA encoding human vitamin D receptor

A R Baker  1 , D P McDonnell, M Hughes, T M Crisp, D J Mangelsdorf, M R Haussler, J W Pike, J Shine, B W O'Malley
Affiliations

Cloning and expression of full-length cDNA encoding human vitamin D receptor

A R Baker et al. Proc Natl Acad Sci U S A. 1988 May.

Abstract

Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of approximately equal to 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

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