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. 2015 Oct 22:8:110-115.
doi: 10.1016/j.btre.2015.10.004. eCollection 2015 Dec.

An investigation on cytotoxic effect of bioactive AgNPs synthesized using Cassia fistula flower extract on breast cancer cell MCF-7

R R Remya  1 , S R Radhika Rajasree  1 , L Aranganathan  1 , T Y Suman  1
Affiliations

An investigation on cytotoxic effect of bioactive AgNPs synthesized using Cassia fistula flower extract on breast cancer cell MCF-7

R R Remya et al. Biotechnol Rep (Amst). .

Abstract

A single step protocol to produce biofunctionalized silver nanoparticles (AgNPs) using the aqueous extract of Cassia fistula flower as "natural factory" was investigated. The reaction between silver ions and aqueous flower extract after the bioreduction process has resulted in the formation of reddish brown color colloidal solution. XRD pattern showed the face centered cubic crystalline structure of AgNPs and exhibited spherical morphology as characterized by FE-SEM. FTIR studies identified different functional groups involved in effective capping of AgNPs. The zeta potential affirmed the phytoreduced AgNPs possess good stability and the size of the particle was measured by DLS. The synthesized AgNPs displayed effective cytotoxic potential against MCF7 and the inhibitory concentration (IC50) was recorded at 7.19 μg/mL. The apoptotic effects of the AgNPs were also confirmed by AO/EB staining. The investigation presents preliminary evidence that biosynthesized AgNPs can be used in the development of novel anticancer drugs.

Keywords: Cassia fistula; Cytotoxicity; Silver nanoparticles.

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Figures

None
Graphical abstract
Fig. 1
Fig. 1
UV spectra of AgNPs synthesized with 1.5 mL of flower extract. The absorption spectra of AgNPs at 422 nm.
Fig. 2
Fig. 2
XRD patterns of biosynthesized AgNPs.
Fig. 3
Fig. 3
FTIR spectra of C. fistulaflower extract.
Fig. 4
Fig. 4
(a) Particle size measurement of AgNPs. (b) Size distribution analysis of synthesized AgNPs.
Fig. 5
Fig. 5
FE-SEM micrographic images of synthesized AgNPs.
Fig. 6
Fig. 6
(a) Cytotoxicity effect of AgNPs against MCF-7 and VERO cell lines at 24 h. (b) AO/EB staining of control live cells (L) showing green fluorescence and apoptotic cells (A) showing red fluorescence.
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