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Review
. 2017 May 27;8(3):169-174.
doi: 10.1080/21541264.2017.1291082. Epub 2017 Feb 8.

Genome-wide characterization of Mediator recruitment, function, and regulation

Sebastian Grünberg  1 , Gabriel E Zentner  2
Affiliations
Review

Genome-wide characterization of Mediator recruitment, function, and regulation

Sebastian Grünberg et al. Transcription. .

Abstract

Mediator is a conserved and essential coactivator complex broadly required for RNA polymerase II (RNAPII) transcription. Recent genome-wide studies of Mediator binding in budding yeast have revealed new insights into the functions of this critical complex and raised new questions about its role in the regulation of gene expression.

Keywords: ChEC-seq; Mediator; Pre-initiation complex; RNA polymerase II; TFIID.

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Figures

Figure 1.
Figure 1.
Mediator binds to ribosomal protein gene UASs, Heatmaps of Mediator ChEC-seq signal (1 min after calcium addition) at RPG TSSs from cells grown in yeast extract-peptone-dextrose (YPD) or glucose complete (GC) medium lacking isoleucine and valine. Heatmaps are normalized to reads per million, log2-transformed, and centered around a value of 10.
See this image and copyright information in PMC

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References

    1. Plaschka C, Lariviere L, Wenzeck L, Seizl M, Hemann M, Tegunov D, Petrotchenko EV, Borchers CH, Baumeister W, Herzog F et al.. Architecture of the RNA polymerase II-Mediator core initiation complex. Nature 2015; 518:376-380; PMID:25652824; https://doi.org/10.1038/nature14229 - DOI - PubMed
    1. Ansari SA, Ganapathi M, Benschop JJ, Holstege FCP, Wade JT, Morse RH. Distinct role of Mediator tail module in regulation of SAGA-dependent, TATA-containing genes in yeast. EMBO J 2012; 31:44-57; PMID:21971086; https://doi.org/10.1038/emboj.2011.362 - DOI - PMC - PubMed
    1. Allen BL, Taatjes DJ. The Mediator complex: a central integrator of transcription. Nat Rev Mol Cell Biol 2015; 16:155-166; PMID:25693131; https://doi.org/10.1038/nrm3951 - DOI - PMC - PubMed
    1. Andrau J-C, van de Pasch L, Lijnzaad P, Bijma T, Koerkamp MG, van de Peppel J, Werner M, Holstege FC. Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA. Mol Cell 2006; 22:179-192; PMID:16630888; https://doi.org/10.1016/j.molcel.2006.03.023 - DOI - PubMed
    1. Zhu X, Wirén M, Sinha I, Rasmussen NN, Linder T, Holmberg S, Ekwall K, Gustafsson CM. Genome-wide occupancy profile of mediator and the Srb8-11 module reveals interactions with coding regions. Mol Cell 2006; 22:169-178; PMID:16630887; https://doi.org/10.1016/j.molcel.2006.03.032 - DOI - PubMed

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