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. 1978 Dec;75(12):6069-72.
doi: 10.1073/pnas.75.12.6069.

Loss of proliferative calcium dependence: simple in vitro indicator of tumorigenicity

S H Swierenga, J F Whitfield, S Karasaki

Loss of proliferative calcium dependence: simple in vitro indicator of tumorigenicity

S H Swierenga et al. Proc Natl Acad Sci U S A. 1978 Dec.

Abstract

The proliferative activities of three lines of "normal" epithelioid rat liver cells and six tumorigenic liver cell lines in the presence of a wide range of calcium concentrations were measured by a simple colony forming assay. The proliferative activities of the normal cells and, to a lesser extent, of the cells of a marginally tumorigenic line were directly proportional to the extracellular calcium concentration. The proliferative activities of the cells of the strongly tumorigenic lines, on the other hand, were either uninfluenced by or inversely proportional to the extracellular calcium concentration. Thus, the proliferative response to the extracellular calcium concentration is a sensitive indicator of the carcinogenic potential of liver cells.

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