Bone Marrow Protein Oxidation in Response to Ionizing Radiation in C57BL/6J Mice

doi:10.3390/proteomes2030291

. 2014 Jun 25;2(3):291-302.

doi: 10.3390/proteomes2030291.

Bone Marrow Protein Oxidation in Response to Ionizing Radiation in C57BL/6J Mice

Yong-Chul Kim¹, Michal Barshishat-Kupper², Elizabeth A McCart³, Gregory P Mueller⁴, Regina M Day⁵

Affiliations

¹ Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. yongchul.kim.ctr@usuhs.edu.
² Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. michalkupper@gmail.com.
³ Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. elizabeth.mccart@usuhs.edu.
⁴ Department of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. gregory.mueller@usuhs.edu.
⁵ Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. regina.day@usuhs.edu.

PMID: 28250382
PMCID: PMC5302751
DOI: 10.3390/proteomes2030291

Bone Marrow Protein Oxidation in Response to Ionizing Radiation in C57BL/6J Mice

Yong-Chul Kim et al. Proteomes. 2014.

. 2014 Jun 25;2(3):291-302.

doi: 10.3390/proteomes2030291.

Authors

Yong-Chul Kim¹, Michal Barshishat-Kupper², Elizabeth A McCart³, Gregory P Mueller⁴, Regina M Day⁵

Affiliations

¹ Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. yongchul.kim.ctr@usuhs.edu.
² Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. michalkupper@gmail.com.
³ Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. elizabeth.mccart@usuhs.edu.
⁴ Department of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. gregory.mueller@usuhs.edu.
⁵ Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA. regina.day@usuhs.edu.

PMID: 28250382
PMCID: PMC5302751
DOI: 10.3390/proteomes2030291

Abstract

The bone marrow is one of the most radio-sensitive tissues. Accidental ionizing radiation exposure can damage mature blood cells and hematopoietic progenitor/stem cells, and mortality can result from hematopoietic insufficiency and infection. Ionizing radiation induces alterations in gene and protein expression in hematopoietic tissue. Here we investigated radiation effects on protein carbonylation, a primary marker for protein oxidative damage. C57BL/6 mice were either sham irradiated or exposed to 7.5 Gy ⁶⁰Co (0.6 Gy/min) total body irradiation. Bone marrow was obtained 24 h post-irradiation. Two dimensional (2-D) gel electrophoresis and Oxyblot immunodetection were used to discover carbonylated proteins, and peptide mass fingerprinting was performed for identification. 2D gels allowed the detection of 13 carbonylated proteins in the bone marrow; seven of these were identified, with two pairs of the same protein. Baseline levels of carbonylation were found in 78 kDa glucose-related protein, heat shock protein cognate 71 KDa, actin, chitinase-like protein 3 (CHI3L1), and carbonic anhydrase 2 (CAII). Radiation increased carbonylation in four proteins, including CHI3L1 and CAII, and induced carbonylation of one additional protein (not identified). Our findings indicate that the profile of specific protein carbonylation in bone marrow is substantially altered by ionizing radiation. Accordingly, protein oxidation may be a mechanism for reduced cell viability.

Keywords: acute radiation syndrome; protein carbonylation; proteomic analysis.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
Two dimensional gel electrophoresis and Oxtblot analysis of sham-irradiated and irradiated bone marrow proteins. C57BL/6 mice were either sham irradiated (control) or exposed to 7.5 Gy TBI. Bone marrow cell lysates were obtained 24 h postirradiation, and protein lysates were combined from 3 mice for one gel. Upper panels: Oxyblot. Lower panels: Coomassie stained gels. Proteins detected by Oxyblot are indicated with numbered arrows. Immunoblots and gels are representative.

See this image and copyright information in PMC

Cited by

Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt⁶⁰ Ionizing Radiation: An Animal Model.
Akeem S, Lukman O, Eltahir K, Fatai O, Abiola B, Khadijat O. Akeem S, et al. Ethiop J Health Sci. 2019 Mar;29(2):195-202. doi: 10.4314/ejhs.v29i2.6. Ethiop J Health Sci. 2019. PMID: 31011267 Free PMC article.
Protracted Oxidative Alterations in the Mechanism of Hematopoietic Acute Radiation Syndrome.
Gorbunov NV, Sharma P. Gorbunov NV, et al. Antioxidants (Basel). 2015 Feb 27;4(1):134-52. doi: 10.3390/antiox4010134. Antioxidants (Basel). 2015. PMID: 26785342 Free PMC article. Review.
Biological Effects of Scattered Versus Scanned Proton Beams on Normal Tissues in Total Body Irradiated Mice: Survival, Genotoxicity, Oxidative Stress and Inflammation.
Chaouni S, Leduc A, Pouzoulet F, De Marzi L, Megnin-Chanet F, Stefan D, Habrand JL, Sichel F, Laurent C. Chaouni S, et al. Antioxidants (Basel). 2020 Nov 24;9(12):1170. doi: 10.3390/antiox9121170. Antioxidants (Basel). 2020. PMID: 33255388 Free PMC article.
Comparison of the Medical Uses and Cellular Effects of High and Low Linear Energy Transfer Radiation.
Russ E, Davis CM, Slaven JE, Bradfield DT, Selwyn RG, Day RM. Russ E, et al. Toxics. 2022 Oct 21;10(10):628. doi: 10.3390/toxics10100628. Toxics. 2022. PMID: 36287908 Free PMC article. Review.
Protein Oxidation in the Lungs of C57BL/6J Mice Following X-Irradiation.
Barshishat-Kupper M, McCart EA, Freedy JG, Tipton AJ, Nagy V, Kim SY, Landauer MR, Mueller GP, Day RM. Barshishat-Kupper M, et al. Proteomes. 2015 Aug 19;3(3):249-265. doi: 10.3390/proteomes3030249. Proteomes. 2015. PMID: 28248270 Free PMC article.

See all "Cited by" articles

References

1. Meder J., Michalowski A. Changes in cellularity and/or weight of mouse hemopoietic Tissues as a measure of acute radiation effects. Arch. Immunol. Ther. Exp. (Warsz) 1980;28:9–18. - PubMed
1. Mauch P., Constine L., Greenberger J., Knospe W., Sullivan J., Liesveld J.L., Deeg H.J. Hematopoietic stem cell compartment: Acute and late effects of radiation therapy and chemotherapy. Int. J. Radiat. Oncol. Biol. Phys. 1995;31:1319–1339. doi: 10.1016/0360-3016(94)00430-S. - DOI - PubMed
1. Davis T.A., Landauer M.R., Mog S.R., Barshishat-Kupper M., Zins S.R., Amare M.F., Day R.M. Timing of captopril administration determines radiation protection or radiation sensitization in a murine model of total body irradiation. Exp. Hematol. 2010;38:270–281. doi: 10.1016/j.exphem.2010.01.004. - DOI - PMC - PubMed
1. Passegue E., Wagers A.J., Giuriato S., Anderson W.C., Weissman I.L. Global analysis of proliferation and cell cycle gene expression in the regulation of hematopoietic stem and progenitor cell fates. J. Exp. Med. 2005;202:1599–1611. doi: 10.1084/jem.20050967. - DOI - PMC - PubMed
1. Cheng T., Rodrigues N., Shen H., Yang Y., Dombkowski D., Sykes M., Scadden D.T. Hematopoietic stem cell quiescence maintained by p21cip1/waf1. Science. 2000;287:1804–1808. - PubMed

LinkOut - more resources

Full Text Sources
Other Literature Sources
- scite Smart Citations

[1] Meder J., Michalowski A. Changes in cellularity and/or weight of mouse hemopoietic Tissues as a measure of acute radiation effects. Arch. Immunol. Ther. Exp. (Warsz) 1980;28:9–18. - PubMed

[2] Meder J., Michalowski A. Changes in cellularity and/or weight of mouse hemopoietic Tissues as a measure of acute radiation effects. Arch. Immunol. Ther. Exp. (Warsz) 1980;28:9–18. - PubMed

[3] Mauch P., Constine L., Greenberger J., Knospe W., Sullivan J., Liesveld J.L., Deeg H.J. Hematopoietic stem cell compartment: Acute and late effects of radiation therapy and chemotherapy. Int. J. Radiat. Oncol. Biol. Phys. 1995;31:1319–1339. doi: 10.1016/0360-3016(94)00430-S. - DOI - PubMed

[4] Mauch P., Constine L., Greenberger J., Knospe W., Sullivan J., Liesveld J.L., Deeg H.J. Hematopoietic stem cell compartment: Acute and late effects of radiation therapy and chemotherapy. Int. J. Radiat. Oncol. Biol. Phys. 1995;31:1319–1339. doi: 10.1016/0360-3016(94)00430-S. - DOI - PubMed

[5] Davis T.A., Landauer M.R., Mog S.R., Barshishat-Kupper M., Zins S.R., Amare M.F., Day R.M. Timing of captopril administration determines radiation protection or radiation sensitization in a murine model of total body irradiation. Exp. Hematol. 2010;38:270–281. doi: 10.1016/j.exphem.2010.01.004. - DOI - PMC - PubMed

[6] Davis T.A., Landauer M.R., Mog S.R., Barshishat-Kupper M., Zins S.R., Amare M.F., Day R.M. Timing of captopril administration determines radiation protection or radiation sensitization in a murine model of total body irradiation. Exp. Hematol. 2010;38:270–281. doi: 10.1016/j.exphem.2010.01.004. - DOI - PMC - PubMed

[7] Passegue E., Wagers A.J., Giuriato S., Anderson W.C., Weissman I.L. Global analysis of proliferation and cell cycle gene expression in the regulation of hematopoietic stem and progenitor cell fates. J. Exp. Med. 2005;202:1599–1611. doi: 10.1084/jem.20050967. - DOI - PMC - PubMed

[8] Passegue E., Wagers A.J., Giuriato S., Anderson W.C., Weissman I.L. Global analysis of proliferation and cell cycle gene expression in the regulation of hematopoietic stem and progenitor cell fates. J. Exp. Med. 2005;202:1599–1611. doi: 10.1084/jem.20050967. - DOI - PMC - PubMed

[9] Cheng T., Rodrigues N., Shen H., Yang Y., Dombkowski D., Sykes M., Scadden D.T. Hematopoietic stem cell quiescence maintained by p21cip1/waf1. Science. 2000;287:1804–1808. - PubMed

[10] Cheng T., Rodrigues N., Shen H., Yang Y., Dombkowski D., Sykes M., Scadden D.T. Hematopoietic stem cell quiescence maintained by p21cip1/waf1. Science. 2000;287:1804–1808. - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Bone Marrow Protein Oxidation in Response to Ionizing Radiation in C57BL/6J Mice

Affiliations

Bone Marrow Protein Oxidation in Response to Ionizing Radiation in C57BL/6J Mice

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

Similar articles

Cited by

References

LinkOut - more resources

Full Text Sources

Other Literature Sources