Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:1556:303-315.
doi: 10.1007/978-1-4939-6771-1_16.

Methods for Observing and Quantifying Muscle Satellite Cell Motility and Invasion In Vitro

Dane K Lund #  1   2 , Patrick McAnulty #  1   3 , Ashley L Siegel #  1   4 , Ddw Cornelison  5
Affiliations

Methods for Observing and Quantifying Muscle Satellite Cell Motility and Invasion In Vitro

Dane K Lund et al. Methods Mol Biol. 2017.

Abstract

Motility and/or chemotaxis of satellite cells has been suggested or observed in multiple in vitro and in vivo contexts. Satellite cell motility also affects the efficiency of muscle regeneration, particularly in the context of engrafted exogenous cells. Consequently, there is keen interest in determining what cell-autonomous and environmental factors influence satellite cell motility and chemotaxis in vitro and in vivo. In addition, the ability of activated satellite cells to relocate in vivo would suggest that they must be able to invade and transit through the extracellular matrix (ECM), which is supported by studies in which alteration or addition of matrix metalloprotease (MMP) activity enhanced the spread of engrafted satellite cells. However, despite its potential importance, analysis of satellite cell motility or invasion quantitatively even in an in vitro setting can be difficult; one of the most powerful techniques for overcoming these difficulties is timelapse microscopy. Identification and longitudinal evaluation of individual cells over time permits not only quantification of variations in motility due to intrinsic or extrinsic factors, it permits observation and analysis of other (frequently unsuspected) cellular activities as well. We describe here three protocols developed in our group for quantitatively analyzing satellite cell motility over time in two dimensions on purified ECM substrates, in three dimensions on a living myofiber, and in three dimensions through an artificial matrix.

Keywords: Cell invasion; Cell motility; Muscle satellite cells; Myofiber culture.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Campion DR (1984) The muscle satellite cell: a review. Int Rev Cytol 87:225–251 - DOI - PubMed
    1. Hawke TJ, Garry DJ (2001) Myogenic satellite cells: physiology to molecular biology. J Appl Physiol 91:534–551 - DOI - PubMed
    1. Shi X, Garry DJ (2006) Muscle stem cells in development, regeneration, and disease. Genes Dev 20:1692–1708 - DOI - PubMed
    1. Peault B, Rudnicki M, Torrente Y, Cossu G, Tremblay JP, Partridge T, Gussoni E, Kunkel LM, Huard J (2007) Stem and progenitor cells in skeletal muscle development, maintenance, and therapy. Mol Ther 15:867–877 - DOI - PubMed
    1. Schmalbruch H (1978) Satellite cells of rat muscles as studied by freeze-fracturing. Anat Rec 191:371–376 - DOI - PubMed

MeSH terms

LinkOut - more resources