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. 2017 Apr;96(4):665-680.
doi: 10.1007/s00277-016-2911-8. Epub 2017 Jan 14.

Comprehensive assessment of peripheral blood TCRβ repertoire in infectious mononucleosis and chronic active EBV infection patients

Shenglin Liu  1 , Qian Zhang  2 , Dongli Huang  3 , Wenli Zhang  2 , Fengluan Zhong  2 , Jia Feng  2 , Xueru Chen  2 , Qingxiang Meng  2 , Xiaofan Chen  1 , Wei Zhang  4 , Hongyu Zhang  5
Affiliations

Comprehensive assessment of peripheral blood TCRβ repertoire in infectious mononucleosis and chronic active EBV infection patients

Shenglin Liu et al. Ann Hematol. 2017 Apr.

Abstract

Epstein-Barr virus (EBV) primary infection is usually asymptomatic, but it sometimes progresses to infectious mononucleosis (IM). Occasionally, some people develop chronic active EBV infection (CAEBV) with underlying immunodeficiency, which belongs to a continuous spectrum of EBV-associated lymphoproliferative disorders (EBV+ LPD) with heterogeneous clinical presentations and high mortality. It has been well established that T cell-mediated immune response plays a critical role in the disease evolution of EBV infection. Recently, high-throughput sequencing of the hypervariable complementarity-determining region 3 (CDR3) segments of the T cell receptor (T cell receptor β (TCRβ)) has emerged as a sensitive approach to assess the T cell repertoire. In this study, we fully characterized the diversity of peripheral blood TCRβ repertoire in IM (n = 6) and CAEBV patients (n = 5) and EBV-seropositive controls (n = 5). Compared with the healthy EBV-seropositive controls, both IM and CAEBV patients demonstrate a significant decrease in peripheral blood TCRβ repertoire diversity, basically, including narrowed repertoire breadth, highly expanded clones, and skewed CDR3 length distribution. However, there is no significant difference between IM and CAEBV patients. Furthermore, we observed some disease-related preferences in TRBV/TRBJ usage and combinations, as well as lots of T cell clones shared by different groups (unique or overlapped) involved in public T cell responses, which provide more detailed insights into the divergent disease evolution.

Keywords: Chronic active EBV infection (CAEBV); Epstein-Barr virus (EBV); Infectious mononucleosis (IM); T cell receptor (TCRβ) repertoire complementarity-determining region 3 (CDR3).

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