Molecular characterization of the Trypanosoma brucei RNA polymerase I and III largest subunit genes
- PMID: 2808366
Molecular characterization of the Trypanosoma brucei RNA polymerase I and III largest subunit genes
Abstract
We have sequenced a 7.1-kilobase fragment of the Trypanosoma brucei RNA polymerase (pol) I largest subunit gene. The 6.9-kilobase transcript from this gene is of roughly equal abundance in bloodstream- and procyclic-form trypanosomes and has a 39-nucleotide trans-spliced leader 290 nucleotides upsteam of the putative initiation codon. The 1781-amino acid trypanosome pol I polypeptide is considerably less similar to its yeast homolog than the trypanosome pol II and pol III polypeptides are to their counterparts in yeast. The yeast and trypanosome pol I polypeptides are distinguished from pol II and pol III largest subunits by two inserts, 100-200 amino acids in length, which are found in the nonconserved portions of the polypeptides. We also report our sequence of the trypanosome pol III largest subunit gene, which agrees with that reported by Köck et al. (Köck, J., Evers, R., and Cornelissen, A.W.C.A. (1988) Nucleic Acids Res. 16, 8753-8772) except for 13 nucleotide differences. Four spliced leader addition sites between 60 and 99 nucleotides upstream of the AUG initiation codon were observed for the 5.4-kilobase transcript from this gene. Four short stretches of amino acid homology between the yeast and trypanosome pol III largest subunit polypeptides were identified which appear to be specific to this class of RNA polymerase; only one short pol I-specific sequence was identified in our comparison of the yeast and trypanosome pol I largest subunit polypeptides.
Similar articles
-
Disruption of largest subunit RNA polymerase II genes in Trypanosoma brucei.Mol Cell Biol. 1993 Jun;13(6):3734-43. doi: 10.1128/mcb.13.6.3734-3743.1993. Mol Cell Biol. 1993. PMID: 8497277 Free PMC article.
-
Structure and sequence of the gene for the largest subunit of trypanosomal RNA polymerase III.Nucleic Acids Res. 1988 Sep 26;16(18):8753-72. doi: 10.1093/nar/16.18.8753. Nucleic Acids Res. 1988. PMID: 3174432 Free PMC article.
-
In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure.Cell. 1989 Mar 10;56(5):815-27. doi: 10.1016/0092-8674(89)90686-7. Cell. 1989. PMID: 2924350
-
Transcription of protein-coding genes in trypanosomes by RNA polymerase I.Annu Rev Microbiol. 1997;51:463-89. doi: 10.1146/annurev.micro.51.1.463. Annu Rev Microbiol. 1997. PMID: 9343357 Review.
-
Evolution of viral DNA-dependent RNA polymerases.Virus Genes. 1995;11(2-3):271-84. doi: 10.1007/BF01728665. Virus Genes. 1995. PMID: 8828152 Review.
Cited by
-
The largest subunit of human RNA polymerase III is closely related to the largest subunit of yeast and trypanosome RNA polymerase III.Genome Res. 1997 Oct;7(10):1006-19. doi: 10.1101/gr.7.10.1006. Genome Res. 1997. PMID: 9331371 Free PMC article.
-
RNA polymerase I transcribes procyclin genes and variant surface glycoprotein gene expression sites in Trypanosoma brucei.Eukaryot Cell. 2003 Jun;2(3):542-51. doi: 10.1128/EC.2.3.542-551.2003. Eukaryot Cell. 2003. PMID: 12796299 Free PMC article.
-
Mutations in the alpha-amanitin conserved domain of the largest subunit of yeast RNA polymerase III affect pausing, RNA cleavage and transcriptional transitions.EMBO J. 1996 Feb 1;15(3):618-29. EMBO J. 1996. PMID: 8599945 Free PMC article.
-
Phylogenetic analysis of the RNA polymerases of Trypanosoma brucei, with special reference to class-specific transcription.Curr Genet. 1990 Dec;18(6):547-51. doi: 10.1007/BF00327026. Curr Genet. 1990. PMID: 2076555
-
Cloning and sequence determination of the Schizosaccharomyces pombe rpb1 gene encoding the largest subunit of RNA polymerase II.Nucleic Acids Res. 1991 Feb 11;19(3):461-8. doi: 10.1093/nar/19.3.461. Nucleic Acids Res. 1991. PMID: 2011520 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
