Photostable and photoswitching fluorescent dyes for super-resolution imaging

doi:10.1007/s00775-016-1435-y

Review

. 2017 Jul;22(5):639-652.

doi: 10.1007/s00775-016-1435-y. Epub 2017 Jan 12.

Photostable and photoswitching fluorescent dyes for super-resolution imaging

Masafumi Minoshima¹, Kazuya Kikuchi^{2

3}

Affiliations

¹ Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan.
² Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan. kkikuchi@mls.eng.osaka-u.ac.jp.
³ Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, 565-0871, Japan. kkikuchi@mls.eng.osaka-u.ac.jp.

PMID: 28083655
DOI: 10.1007/s00775-016-1435-y

Review

Photostable and photoswitching fluorescent dyes for super-resolution imaging

Masafumi Minoshima et al. J Biol Inorg Chem. 2017 Jul.

. 2017 Jul;22(5):639-652.

doi: 10.1007/s00775-016-1435-y. Epub 2017 Jan 12.

Authors

Masafumi Minoshima¹, Kazuya Kikuchi^{2

3}

Affiliations

¹ Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan.
² Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan. kkikuchi@mls.eng.osaka-u.ac.jp.
³ Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, 565-0871, Japan. kkikuchi@mls.eng.osaka-u.ac.jp.

PMID: 28083655
DOI: 10.1007/s00775-016-1435-y

Abstract

Super-resolution fluorescence microscopy is a recently developed imaging tool for biological researches. Several methods have been developed for detection of fluorescence signals from molecules in a subdiffraction-limited area, breaking the diffraction limit of the conventional optical microscopies and allowing visualization of detailed macromolecular structures in cells. As objectives are exposed to intense laser in the optical systems, fluorophores for super-resolution microscopy must be tolerated even under severe light irradiation conditions. The fluorophores must also be photoactivatable and photoswitchable for single-molecule localization-based super-resolution microscopy, because the number of active fluorophores must be controlled by light irradiation. This has led to growing interest in these properties in the development of fluorophores. In this mini-review, we focus on the development of photostable and photoswitching fluorescent dyes for super-resolution microscopy. We introduce recent efforts, including improvement of fluorophore photostability and control of photoswitching behaviors of fluorophores based on photochemical and photophysical processes. Understanding and manipulation of chemical reactions in excited fluorophores can develop highly photostable and efficiently photoswitchable fluorophores that are suitable for super-resolution imaging applications.

Keywords: Fluorophores; Photoactivation; Photostability; Photoswitching; Super-resolution microscopy.

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[1] J Am Chem Soc. 2015 Apr 15;137(14):4602-5 - PubMed

[2] J Am Chem Soc. 2015 Apr 15;137(14):4602-5 - PubMed

[3] Nat Methods. 2015 Sep;12(9):827-30 - PubMed

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Photostable and photoswitching fluorescent dyes for super-resolution imaging

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Photostable and photoswitching fluorescent dyes for super-resolution imaging

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