Three-dimensional imaging flow cytometry through light-sheet fluorescence microscopy
- PMID: 28075531
- DOI: 10.1002/cyto.a.23046
Three-dimensional imaging flow cytometry through light-sheet fluorescence microscopy
Abstract
Flow cytometry is the tool of choice for high-speed acquisition and analysis of large cell populations, with the tradeoff of lacking intracellular spatial information. Although in the last decades flow cytometry systems that can actually acquire two-dimensional spatial information were developed, some of the limitations remained though, namely constrains related to sample size and lack of depth or dynamic information. The combination of fluidics and light-sheet illumination has the potential to address these limitations. By having cells travelling with the flowing sheath one can, in a controlled fashion, force them at constant speed through the light-sheet enabling the synchronized acquisition of several optical sections, that is, three-dimensional imaging. This approach has already been used for imaging cellular spheroids, plankton, and zebra-fish embryos. In this review, we discuss the known solutions and standing challenges of performing three-dimensional high-throughput imaging of multicellular biological models using fluidics, while retaining cell and organelle-level resolution. © 2017 International Society for Advancement of Cytometry.
Keywords: high throughput microscopy; imaging flow cytometry; light sheet fluorescence microscopy.
© 2017 International Society for Advancement of Cytometry.
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