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. 2017 Nov;35(6):608-615.
doi: 10.1007/s00774-016-0799-y. Epub 2016 Nov 21.

MiR-5100 promotes osteogenic differentiation by targeting Tob2

Huaxin Wang  1   2 , Yazhou Cui  2 , Jing Luan  2 , Xiaoyan Zhou  2 , Chengzhi Li  2 , Haiying Li  2 , Liang Shi  2 , Jinxiang Han  3
Affiliations

MiR-5100 promotes osteogenic differentiation by targeting Tob2

Huaxin Wang et al. J Bone Miner Metab. 2017 Nov.

Abstract

MicroRNAs have emerged as pivotal regulators in various physiological and pathological processes, including osteogenesis. Here we discuss the contribution of miR-5100 to osteoblast differentiation and mineralization. We found that miR-5100 was upregulated during osteoblast differentiation in ST2 and MC3T3-E1 cells. Next, we verified that miR-5100 can promote osteogenic differentiation with gain-of-function and loss-of-function experiments. Target prediction analysis and experimental validation demonstrated that Tob2, which acts as a negative regulator of osteogenesis, was negatively regulated by miR-5100. Furthermore, we confirmed that the important bone-related transcription factor osterix, which can be degraded by binding to Tob2, was influenced by miR-5100 during osteoblast differentiation. Collectively, our results revealed a new molecular mechanism that fine-tunes osteoblast differentiation through miR-5100/Tob2/osterix networks.

Keywords: Mineralization; Osteoblast differentiation; Tob2; miR-5100.

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