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. 2017 Jan;14(1):53-56.
doi: 10.1038/nmeth.4074. Epub 2016 Nov 21.

mScarlet: a bright monomeric red fluorescent protein for cellular imaging

Daphne S Bindels  1 , Lindsay Haarbosch  1 , Laura van Weeren  1 , Marten Postma  1 , Katrin E Wiese  1 , Marieke Mastop  1 , Sylvain Aumonier  2   3 , Guillaume Gotthard  2   3 , Antoine Royant  2   3 , Mark A Hink  1 , Theodorus W J Gadella Jr  1
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mScarlet: a bright monomeric red fluorescent protein for cellular imaging

Daphne S Bindels et al. Nat Methods. 2017 Jan.

Abstract

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.

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