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. 2017;38(3):299-307.
doi: 10.1080/15321819.2016.1260587. Epub 2016 Nov 17.

Immunoassay and molecular methods to investigate DNA methylation changes in peripheral blood mononuclear cells in HIV infected patients on cART

Adelina Rosca  1   2 , Gabriela Anton  2 , Luminita Ene  3 , Iulia Iancu  2 , Aura Temereanca  1   2 , Cristian L Achim  4 , Simona M Ruta  1   2
Affiliations

Immunoassay and molecular methods to investigate DNA methylation changes in peripheral blood mononuclear cells in HIV infected patients on cART

Adelina Rosca et al. J Immunoassay Immunochem. 2017.

Abstract

This study aimed to investigate the influence of antiretroviral therapy on methylation markers, in a group of HIV infected, heavily treated patients. Immune and molecular methods were used to investigate potential changes in methylation profile in DNA isolated from peripheral blood mononuclear cells collected from antiretroviral-experienced HIV infected patients and healthy controls. The percentage of 5-methylcytosine was inversely correlated with proviral DNA and active replication while DNMT1 (p = 0.01) and DNMT3A (p = 0.004) independently correlated with active viral replication. DNMT3A expression increased with total treatment duration (p = 0.03), number of antiretroviral drugs ever used (p = 0.003), and cumulative exposure to protease inhibitors (p = 0.02) even in currently HIV undetectable patients.

Keywords: DNA methylation; DNMT1; DNMT3A; HIV; PBMC; antiretroviral treatment; epigenetics.

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Figures

Figure 1
Figure 1
Expression of enzymes involved in maintaining methylation patterns in HIV patients. a. Box plot representing DNMT3A and DNMT1 expression in patients with detectable (HIV VL DET), n=24 and undetectable (HIV VL ND), n=57 HIV plasma viral load. Differences between groups were analyzed by Mann-Whitney test. b. Box plot representing DNMT3A and DNMT1 relative expression in patients treated for more (n=41) and less (n=40) than group median ART duration (122.87 months). Differences between groups were analyzed by Mann-Whitney test.
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