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. 2017 Mar;131(3):281-289.
doi: 10.1007/s11120-016-0320-4. Epub 2016 Nov 5.

Effects of co-overexpression of the genes of Rubisco and transketolase on photosynthesis in rice

Yuji Suzuki  1   2   3 , Eri Kondo  4 , Amane Makino  4   5
Affiliations

Effects of co-overexpression of the genes of Rubisco and transketolase on photosynthesis in rice

Yuji Suzuki et al. Photosynth Res. 2017 Mar.

Abstract

Metabolome analyses have indicated an accumulation of sedoheptulose 7-phosphate in transgenic rice plants with overproduction of Rubisco (Suzuki et al. in Plant Cell Environ 35:1369-1379, 2012. doi: 10.1111/j.1365-3040.2012.02494.x ). Since Rubisco overproduction did not quantitatively enhance photosynthesis even under CO2-limited conditions, it is suspected that such an accumulation of sedoheptulose 7-phosphate hampers the improvement of photosynthetic capacity. In the present study, the gene of transketolase, which is involved in the metabolism of sedoheptulose 7-phosphate, was co-overexpressed with the Rubisco small subunit gene in rice. Rubisco and transketolase were successfully overproduced in comparison with those in wild-type plants by 35-53 and 39-84 %, respectively. These changes in the amounts of the proteins were associated with those of the mRNA levels. However, the rate of CO2 assimilation under high irradiance and different [CO2] did not differ between co-overexpressed plants and wild-type plants. Thus, co-overproduction of Rubisco and transketolase did not improve photosynthesis in rice. Transketolase was probably not a limiting factor of photosynthesis as overproduction of transketolase alone by 80-94 % did not affect photosynthesis.

Keywords: CO2 assimilation; Overproduction; Rice; Rubisco; Transketolase.

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