Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:1510:339-351.
doi: 10.1007/978-1-4939-6527-4_25.

Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques

Tobias Wagner  1 , Maren Godmann  1 , Thorsten Heinzel  2
Affiliations

Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques

Tobias Wagner et al. Methods Mol Biol. 2017.

Abstract

Histone deacetylases (HDACs) are controlling dynamic protein acetylation by removing acetyl moieties from lysine. Histone deacetylases themselves are regulated on the posttranslational level, including modifications with small ubiquitin-like modifier (SUMO) proteins. Detecting SUMO modifications of deacetylases by immunoblotting is technically challenging due to the typically low ratio of the modified compared to the unmodified species. Here, we describe a set of methods for the detection of endogenous sumoylated HDACs by immunoprecipitation and immunoblotting techniques.

Keywords: Epigenetics; HDAC; Histone deacetylases; Immunoblotting; Immunoprecipitation; Posttranslational modification; SUMO; Sumoylation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

MeSH terms

LinkOut - more resources