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. 2016 Nov 10;539(7628):299-303.
doi: 10.1038/nature20104. Epub 2016 Oct 17.

Reconstitution in vitro of the entire cycle of the mouse female germ line

Orie Hikabe  1 , Nobuhiko Hamazaki  1 , Go Nagamatsu  1 , Yayoi Obata  2 , Yuji Hirao  3 , Norio Hamada  1   4 , So Shimamoto  1 , Takuya Imamura  1 , Kinichi Nakashima  1 , Mitinori Saitou  5   6   7   8 , Katsuhiko Hayashi  1   9
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Free article

Reconstitution in vitro of the entire cycle of the mouse female germ line

Orie Hikabe et al. Nature. .
Free article

Abstract

The female germ line undergoes a unique sequence of differentiation processes that confers totipotency to the egg. The reconstitution of these events in vitro using pluripotent stem cells is a key achievement in reproductive biology and regenerative medicine. Here we report successful reconstitution in vitro of the entire process of oogenesis from mouse pluripotent stem cells. Fully potent mature oocytes were generated in culture from embryonic stem cells and from induced pluripotent stem cells derived from both embryonic fibroblasts and adult tail tip fibroblasts. Moreover, pluripotent stem cell lines were re-derived from the eggs that were generated in vitro, thereby reconstituting the full female germline cycle in a dish. This culture system will provide a platform for elucidating the molecular mechanisms underlying totipotency and the production of oocytes of other mammalian species in culture.

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Comment in

  • Are human oocytes from stem cells next?
    Smitz JE, Gilchrist RB. Smitz JE, et al. Nat Biotechnol. 2016 Dec 7;34(12):1247-1248. doi: 10.1038/nbt.3742. Nat Biotechnol. 2016. PMID: 27926709 No abstract available.
  • Recreating the Female Germline In Vitro.
    Gerhardt K. Gerhardt K. Biol Reprod. 2016 Dec;95(6):133. doi: 10.1095/biolreprod.116.146613. Epub 2016 Nov 2. Biol Reprod. 2016. PMID: 28007694 No abstract available.

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