Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2017:1511:13-28.
doi: 10.1007/978-1-4939-6533-5_2.

Approaches to Characterize Organelle, Compartment, or Structure Purity

Stefanie J Mueller  1 , Sebastian N W Hoernstein  1 , Ralf Reski  2   3   4   5
Affiliations
Review

Approaches to Characterize Organelle, Compartment, or Structure Purity

Stefanie J Mueller et al. Methods Mol Biol. 2017.

Abstract

The function of subcellular structures is defined by their specific sets of proteins, making subcellular protein localization one of the most important topics in organelle research. To date, many organelle proteomics workflows involve the (partial) purification of the desired subcellular structure and the subsequent analysis of the proteome using tandem mass spectrometry (MS/MS). This chapter gives an overview of the methods that have been used to assay the purity and enrichment of subcellular structures, with an emphasis on quantitative proteomics using differently enriched subcellular fractions. We introduce large-scale-based criteria for assignment of proteins to subcellular structures and describe in detail the use of 15N metabolic labeling in moss to characterize plastid and mitochondrial proteomes.

Keywords: Compartment marker; Density gradient purification; Metabolic labeling; Mitochondria; Physcomitrella patens; Plastid; Quantitative proteomics.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

Publication types

MeSH terms

LinkOut - more resources