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. 2016 Oct;12(4):2741-2746.
doi: 10.3892/etm.2016.3674. Epub 2016 Sep 6.

Tanshinone IIA pretreatment attenuates ischemia/reperfusion-induced renal injury

Yan-Mei Xu  1 , Guo-Hua Ding  1 , Jie Huang  1 , Yan Xiong  1
Affiliations

Tanshinone IIA pretreatment attenuates ischemia/reperfusion-induced renal injury

Yan-Mei Xu et al. Exp Ther Med. 2016 Oct.

Abstract

Tanshinone IIA is a chemical compound extracted from the root of traditional Chinese herb Salvia miltiorrhiza Bunge. Tanshinone IIA has been suggested to possess anti-inflammatory activity and antioxidizing capability. Recently, accumulating results have indicated the antitumor activity of tanshinone IIA; thus, it has attracted increasing attention. In addition, tanshinone IIA has been indicated to attenuate ischemia/reperfusion induced renal injury (I/RIRI); however, little is known regarding the underlying mechanisms involved in this process. In the present study an I/RIRI rat model was used to analyze the effects of tanshinone IIA on myeloperoxidase (MPO), TNF-α and IL-6 activities using ELISA kits. Furthermore, macrophage migration inhibitory factor (MIF), cleaved caspase-3, B-cell lymphoma 2 (Bcl-2) and p38 mitogen-activated protein kinase (MAPK) protein expression levels were evaluated using western blot analysis. The results indicated that tanshinone IIA protected renal function in I/RIRI rats. ELISA demonstrated that tanshinone IIA significantly reduced MIF, TNF-α and IL-6 activities in I/RIRI rats. Western blot analysis showed that tanshinone IIA significantly suppressed MIF, cleaved caspase-3 and p38 MAPK protein expression levels in I/RIRI rats. The present results suggest that tanshinone IIA pretreatment attenuates I/RIRI via the downregulation of MPO expression, inflammation, MIF, cleaved caspase-3 and p38 MAPK.

Keywords: inflammation; ischemia/reperfusion induced renal injury; myeloperoxidase; tanshinone IIA.

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Figures

Figure 1.
Figure 1.
Chemical structure of tanshinone IIA.
Figure 2.
Figure 2.
Effect of tanshinone IIA on (A) blood urea nitrogen and (B) creatinine levels in ischemia/reperfusion induced renal injury (I/RIRI) rat. #P<0.01 vs. Control group; *P<0.01 vs. I/RIRI model group. Control, control group; Model, I/RIRI model group; Tan, tanshinone IIA group.
Figure 3.
Figure 3.
Effect of tanshinone IIA on MPO activity. #P<0.01 vs. Control group; *P<0.01 vs. ischemia/reperfusion induced renal injury (I/RIRI) model group. MPO, myeloperoxidase; Control, control group; Model, I/RIRI model group; Tan, tanshinone IIA group.
Figure 4.
Figure 4.
Effect of tanshinone IIA on (A) TNF-α and (B) IL-6 activities in ischemia/reperfusion induced renal injury (I/RIRI) rats. #P<0.01 vs. Control group; *P<0.01 vs. I/RIRI model group. TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; Control, control group; Model, I/RIRI model group; Tan, tanshinone IIA group.
Figure 5.
Figure 5.
Effect of tanshinone IIA on MIF activity. #P<0.01 vs. Control group; *P<0.01 vs. ischemia/reperfusion induced renal injury (I/RIRI) model group. MIF, migration inhibitory factor; Control, control group; Model, I/RIRI model group; Tan, tanshinone IIA group.
Figure 6.
Figure 6.
Effect of tanshinone IIA on cleaved caspase-3. Effect of tanshinone IIA on cleaved caspase-3 protein expression using (A) western blotting analysis, and (B) calculated cleaved caspase-3 protein expression. #P<0.01 vs. Control group; *P<0.01 vs. ischemia/reperfusion induced renal injury (I/RIRI) model group. Control, control group; Model, I/RIRI model group; Tan, tanshinone IIA group.
Figure 7.
Figure 7.
Effect of tanshinone IIA on Bcl-2 protein expression. Effect of tanshinone IIA on Bcl-2 protein expression using (A) western blotting analysis, and (B) calculated Bcl-2 protein expression. #P<0.01 vs. Control group; *P<0.01 vs. ischemia/reperfusion induced renal injury (I/RIRI) model group. Control, control group; Model, I/RIRI model group; Tan, tanshinone IIA group.
Figure 8.
Figure 8.
Effect of tanshinone IIA on p-p38 MAPK protein expression. Effect of tanshinone IIA on p-p38 MAPK protein expression using (A) western blotting analysis, and (B) calculated p-p38 MAPK protein expression. #P<0.01 vs. Control group; *P<0.01 vs. ischemia/reperfusion induced renal injury (I/RIRI) model group. Control, control group; Model, I/RIRI model group; Tan, tanshinone IIA group.
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