Cerebral Cortex Electroporation to Study Projection Neuron Migration

doi:10.1002/cpns.13

. 2016 Oct 3:77:2.26.1-2.26.18.

doi: 10.1002/cpns.13.

Cerebral Cortex Electroporation to Study Projection Neuron Migration

Emilie Pacary^{1

2}, François Guillemot³

Affiliations

¹ INSERM U1215, Neurocentre Magendie, Bordeaux, France.
² Université de Bordeaux, Bordeaux, France.
³ The Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.

PMID: 27696363
DOI: 10.1002/cpns.13

Cerebral Cortex Electroporation to Study Projection Neuron Migration

Emilie Pacary et al. Curr Protoc Neurosci. 2016.

. 2016 Oct 3:77:2.26.1-2.26.18.

doi: 10.1002/cpns.13.

Authors

Emilie Pacary^{1

2}, François Guillemot³

Affiliations

¹ INSERM U1215, Neurocentre Magendie, Bordeaux, France.
² Université de Bordeaux, Bordeaux, France.
³ The Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.

PMID: 27696363
DOI: 10.1002/cpns.13

Abstract

Brain electroporation is a rapid and powerful approach to study neuronal development. In particular, this technique has become a method of choice for studying the process of radial migration of projection neurons in the embryonic cerebral cortex. This method has considerably helped to describe in detail the different steps of radial migration and to characterize the molecular mechanisms controlling this process. Delineating the complexities of neuronal migration is critical to our understanding not only of normal cerebral cortex formation but also of neurodevelopmental disorders resulting from neuronal migration defects. Here, we describe in detail the protocols to perform in utero or ex vivo electroporation of progenitor cells in the ventricular zone of the cerebral cortex with the aim of studying the process of radial migration of projection neurons during embryonic development. © 2016 by John Wiley & Sons, Inc.

Keywords: cerebral cortex; electroporation; migration; neuron.

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References

Literature Cited

1. Evsyukova, I., Plestant, C., and Anton, E.S. 2013. Integrative mechanisms of oriented neuronal migration in the developing brain. Annu. Rev. Cell Dev. Biol. 29:299-353. doi: 10.1146/annurev-cellbio-101512-122400.
1. Fukuchi-Shimogori, T. and Grove, E.A. 2001. Neocortex patterning by the secreted signaling molecule FGF8. Science 294:1071-1074. doi: 10.1126/science.1064252.
1. McManus, M.F. and Golden, J.A. 2005. Neuronal migration in developmental disorders. J. Child Neurol. 20:280-286. doi: 10.1177/08830738050200040301.
1. Muramatsu, T., Mizutani, Y., Ohmori, Y., and Okumura, J. 1997. Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem. Biophys. Res. Commun. 230:376-380. doi: 10.1006/bbrc.1996.5882.
1. Nadarajah, B., Brunstrom, J.E., Grutzendler, J., Wong, R.O., and Pearlman, A.L. 2001. Two modes of radial migration in early development of the cerebral cortex. Nat. Neurosci. 4:143-150. doi: 10.1038/83967.

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[1] Evsyukova, I., Plestant, C., and Anton, E.S. 2013. Integrative mechanisms of oriented neuronal migration in the developing brain. Annu. Rev. Cell Dev. Biol. 29:299-353. doi: 10.1146/annurev-cellbio-101512-122400.

[2] Evsyukova, I., Plestant, C., and Anton, E.S. 2013. Integrative mechanisms of oriented neuronal migration in the developing brain. Annu. Rev. Cell Dev. Biol. 29:299-353. doi: 10.1146/annurev-cellbio-101512-122400.

[3] Fukuchi-Shimogori, T. and Grove, E.A. 2001. Neocortex patterning by the secreted signaling molecule FGF8. Science 294:1071-1074. doi: 10.1126/science.1064252.

[4] Fukuchi-Shimogori, T. and Grove, E.A. 2001. Neocortex patterning by the secreted signaling molecule FGF8. Science 294:1071-1074. doi: 10.1126/science.1064252.

[5] McManus, M.F. and Golden, J.A. 2005. Neuronal migration in developmental disorders. J. Child Neurol. 20:280-286. doi: 10.1177/08830738050200040301.

[6] McManus, M.F. and Golden, J.A. 2005. Neuronal migration in developmental disorders. J. Child Neurol. 20:280-286. doi: 10.1177/08830738050200040301.

[7] Muramatsu, T., Mizutani, Y., Ohmori, Y., and Okumura, J. 1997. Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem. Biophys. Res. Commun. 230:376-380. doi: 10.1006/bbrc.1996.5882.

[8] Muramatsu, T., Mizutani, Y., Ohmori, Y., and Okumura, J. 1997. Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem. Biophys. Res. Commun. 230:376-380. doi: 10.1006/bbrc.1996.5882.

[9] Nadarajah, B., Brunstrom, J.E., Grutzendler, J., Wong, R.O., and Pearlman, A.L. 2001. Two modes of radial migration in early development of the cerebral cortex. Nat. Neurosci. 4:143-150. doi: 10.1038/83967.

[10] Nadarajah, B., Brunstrom, J.E., Grutzendler, J., Wong, R.O., and Pearlman, A.L. 2001. Two modes of radial migration in early development of the cerebral cortex. Nat. Neurosci. 4:143-150. doi: 10.1038/83967.

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Cerebral Cortex Electroporation to Study Projection Neuron Migration

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Cerebral Cortex Electroporation to Study Projection Neuron Migration

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