α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

doi:10.1007/s10787-016-0280-5

. 2016 Oct;24(5):277-286.

doi: 10.1007/s10787-016-0280-5. Epub 2016 Sep 26.

α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

Affiliations

¹ Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
² Department of Pharmaceutical Sciences, FHMSIASM SHIATS-Deemed University (Formerly Allahabad Agriculture Institute), Naini, Allahabad, 211007, UP, India.
³ Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
⁴ Department of Biotechnology, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
⁵ Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India. gauravpharm@hotmail.com.

PMID: 27671329
DOI: 10.1007/s10787-016-0280-5

α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

Asha Rani et al. Inflammopharmacology. 2016 Oct.

. 2016 Oct;24(5):277-286.

doi: 10.1007/s10787-016-0280-5. Epub 2016 Sep 26.

Authors

Affiliations

¹ Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
² Department of Pharmaceutical Sciences, FHMSIASM SHIATS-Deemed University (Formerly Allahabad Agriculture Institute), Naini, Allahabad, 211007, UP, India.
³ Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
⁴ Department of Biotechnology, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India.
⁵ Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidyavihar, Raebareli road, Lucknow, 226 025, UP, India. gauravpharm@hotmail.com.

PMID: 27671329
DOI: 10.1007/s10787-016-0280-5

Abstract

This study was undertaken to investigate the effect of α-chymotrypsin on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized into four groups (six animals in each). Group I (sham control 0.9 % normal saline p.o.); Group II (toxic control, MNU 47 mg/kg, i.v.); Group III (α-chymotrypsin, 5 mg/kg, p.o.); Group IV (α-chymotrypsin, 10 mg/kg p.o.). Toxicity was induced by single i.v. injection of MNU followed by α-chymotrypsin supplementation therapy for 100 days. MNU treatment was evident with increased alveolar bud count, differentiation score, upregulated inflammatory enzymes markers (COX, LOX and NO) antioxidative stress markers (TBARs, SOD, catalase and GSH).MNU associated toxicity was also ascertained by PGP 9.5 and NF-κB expression in the mammary gland tissue followed by FAME analysis for fatty acid profiling. α-chymotrypsin afforded significant protection against the deleterious effects of MNU.

Keywords: FAME; Mammary gland; NF-κB p65; Oxidative stress; PGP 9.5; α-Chymotrypsin.

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[1] Mol Cell Endocrinol. 1985 Oct;42(3):207-14 - PubMed

[2] Mol Cell Endocrinol. 1985 Oct;42(3):207-14 - PubMed

[3] Neuropsychopharmacology. 2005 Jan;30(1):80-9 - PubMed

[4] Neuropsychopharmacology. 2005 Jan;30(1):80-9 - PubMed

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[9] Interdiscip Toxicol. 2013 Dec;6(4):222-7 - PubMed

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α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

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α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats

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