doi: 10.1007/978-1-4939-6380-5_10.
Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP)
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- PMID: 27659979
- DOI: 10.1007/978-1-4939-6380-5_10
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Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP)
Methods Mol Biol.
2016.
Abstract
ChIRP is a novel and easy-to-use technique for studying long noncoding RNA (lncRNA)-chromatin interactions. RNA and chromatin are cross-linked in vivo using formaldehyde or glutaraldehyde, and purified using biotinylated antisense oligonucleotides that hybridize to the target RNA. Co-precipitated DNA is then purified and analyzed by quantitative PCR (qPCR) or high-throughput sequencing.
Keywords: Chromatin; Cross-linking; High-throughput sequencing; lncRNA.
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