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. 2016 Nov;16(11):703-708.
doi: 10.1089/vbz.2016.1961. Epub 2016 Sep 22.

Phenogenotypic Characterization of Escherichia coli O157:H7 Strains Isolated from Cattle at Slaughter

Toomas Kramarenko  1   2 , Mati Roasto  1 , Mihkel Mäesaar  1   2 , Antonella Maugliani  3 , Rosangela Tozzoli  3 , Kadrin Meremäe  1 , Terje Elias  1 , Maiu Kuningas  2
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Phenogenotypic Characterization of Escherichia coli O157:H7 Strains Isolated from Cattle at Slaughter

Toomas Kramarenko et al. Vector Borne Zoonotic Dis. 2016 Nov.

Abstract

Shiga toxin-producing Escherichia coli (STEC) O157:H7 was isolated from 30 (4%) of 744 cattle hide swab samples collected at Estonian slaughterhouses within a 3-year monitoring program of zoonotic pathogens. The isolates were characterized by determining the presence of STEC main virulence factors, the antimicrobial resistance profiles, and the genetic relatedness by pulsed-field gel electrophoresis (PFGE). Thirteen strains carried the stx2 gene alone and 17 both the stx1 and stx2 genes. The most frequently detected stx subtype was stx2c, occurring alone (n = 12) or in combination with subtype stx1a (n = 13). All isolates harbored the intimin-coding eae gene and produced enterohemolysin. Twelve isolates (40%) showed resistance to at least one of the 14 antimicrobials and the isolates were predominantly resistant to streptomycin, sulfamethoxazole, and ampicillin. No extended-spectrum beta-lactamase-producing isolates were detected. PFGE characterization of the isolates showed an overall similarity higher than 75%, and four clusters based on 100% similarity were revealed.

Keywords: Escherichia coli O157:H7; cattle; slaughterhouse; zoonotic agents.

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