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Review
. 2017 Jan 2;8(1):11-16.
doi: 10.1080/19491034.2016.1236167. Epub 2016 Sep 20.

Plant nucleolar DNA: Green light shed on the role of Nucleolin in genome organization

Claire Picart  1   2 , Frédéric Pontvianne  1   2
Affiliations
Review

Plant nucleolar DNA: Green light shed on the role of Nucleolin in genome organization

Claire Picart et al. Nucleus. .

Abstract

The nucleolus forms as a consequence of ribosome biogenesis, but it is also implicated in other cell functions. The identification of nucleolus-associated chromatin domains (NADs) in animal and plant cells revealed the presence of DNA sequences other than rRNA genes in and around the nucleolus. NADs display repressive chromatin signatures and harbour repetitive DNA, but also tRNA genes and RNA polymerase II-transcribed genes. Furthermore, the identification of NADs revealed a specific function of the nucleolus and the protein Nucleolin 1 (NUC1) in telomere biology. Here, we discuss the significance of these data with regard to nucleolar structure and to the role of the nucleolus and NUC1 in global genome organization and stability.

Keywords: genome stability; heterochromatin; nuclear architecture; nucleolus; nucleolus-associated chromatin domains.

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Figures

Figure 1.
Figure 1.
Chromatin and Fibrillarin distribution in WT and nuc1 nuclei Confocal images of 5 nuclei from WT (A) or nuc1 mutant (B) expressing the Fibrillarin fused to YFP (green). DNA is labeled by DAPI (blue) and scale bars represent 5 μm.
Figure 2.
Figure 2.
NUC1 and NUC2 proteins organization A. Schematic representation of NUC1 (AT1G48920) and NUC2 (AT3G18610) proteins from A. thaliana. B. Amino acid sequence alignment of the R/G rich domains of NUC1 and NUC2. C. Disorder profile plots of NUC1 and NUC2 amino acid sequences, obtained with the prediction software PrDOS. The black lines correspond to the threshold for a 2% prediction false rate.
See this image and copyright information in PMC

Erratum for

  • Extra View to: Pontvianne F, Carpentier MC, Durut N, Pavlištová V, Jaške K, Schořová Š, Parrinello H, Rohmer M, Pikaard CS, Fojtová M, Fajkus J, Sáez-Vásquez J. Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome. PCell Rep. 2016. 16(6):1574–87. http://dx.doi.org/10.1016/j.celrep.2016.07

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