Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2015 Oct 16;4(4):454-73.
doi: 10.3390/microarrays4040454.

Genomic-Wide Analysis with Microarrays in Human Oncology

Kenichi Inaoka  1 , Yoshikuni Inokawa  2 , Shuji Nomoto  3
Affiliations
Review

Genomic-Wide Analysis with Microarrays in Human Oncology

Kenichi Inaoka et al. Microarrays (Basel). .

Abstract

DNA microarray technologies have advanced rapidly and had a profound impact on examining gene expression on a genomic scale in research. This review discusses the history and development of microarray and DNA chip devices, and specific microarrays are described along with their methods and applications. In particular, microarrays have detected many novel cancer-related genes by comparing cancer tissues and non-cancerous tissues in oncological research. Recently, new methods have been in development, such as the double-combination array and triple-combination array, which allow more effective analysis of gene expression and epigenetic changes. Analysis of gene expression alterations in precancerous regions compared with normal regions and array analysis in drug-resistance cancer tissues are also successfully performed. Compared with next-generation sequencing, a similar method of genome analysis, several important differences distinguish these techniques and their applications. Development of novel microarray technologies is expected to contribute to further cancer research.

Keywords: combination array; gene expression; microarray.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Fodor S.P., Read J.L., Pirrung M.C., Stryer L., Lu A.T., Solas D. Light-directed, spatially addressable parallel chemical synthesis. Science. 1991;251:767–773. doi: 10.1126/science.1990438. - DOI - PubMed
    1. Pease A.C., Solas D., Sullivan E.J., Cronin M.T., Holmes C.P., Fodor S.P. Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc. Natl. Acad. Sci. USA. 1994;91:5022–5026. doi: 10.1073/pnas.91.11.5022. - DOI - PMC - PubMed
    1. Schena M. Genome analysis with gene expression microarrays. Bioessays. 1996;18:427–431. doi: 10.1002/bies.950180513. - DOI - PubMed
    1. Schena M., Shalon D., Heller R., Chai A., Brown P.O., Davis R.W. Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes. Proc. Natl. Acad. Sci. USA. 1996;93:10614–10619. doi: 10.1073/pnas.93.20.10614. - DOI - PMC - PubMed
    1. Shalon D., Smith S.J., Brown P.O. A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res. 1996;6:639–645. doi: 10.1101/gr.6.7.639. - DOI - PubMed

LinkOut - more resources