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. 2016 Oct 11;115(8):1008-1014.
doi: 10.1038/bjc.2016.261. Epub 2016 Aug 23.

Virome characterisation from Guthrie cards in children who later developed acute lymphoblastic leukaemia

G Bogdanovic  1   2 , C Pou  3 , M Barrientos-Somarribas  3 , A Bjerkner  1   2 , E Honkaniemi  4   5 , T Allander  1   2 , B Andersson  3 , B Gustafsson  5   6
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Virome characterisation from Guthrie cards in children who later developed acute lymphoblastic leukaemia

G Bogdanovic et al. Br J Cancer. .

Abstract

Background: Some childhood acute lymphoblastic leukaemias (ALL) can be traced back to a prenatal origin, where a virus infection could be involved in the first pre-leukaemic clone development. The DNA virome of 95 children who later developed ALL was characterised from neonatal blood spots (NBS) using unbiased next-generation sequencing (NGS) and compared with the virome of 95 non-ALL controls.

Methods: DNA was individually extracted from the ALL-patients and controls, pooled, randomly amplified and sequenced using the Illumina MiSeq Sequencing System.

Results: Virus-like sequences identified in both groups mapped to human endogenous retroviruses and propionibacterium phage, considered a part of the normal microbial flora. Potential pathogens human herpesvirus type 6 (HHV-6) and parvovirus B19 were also identified, but only few samples in both ALL and controls tested positive by PCR follow-up.

Conclusions: Unbiased NGS was employed to search for DNA from potential infectious agents in neonatal samples of children who later developed ALL. Although several viral candidates were identified in the NBS samples, further investigation by PCR suggested that these viruses did not have a major role in ALL development.

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Figures

Figure 1
Figure 1
Visualisation of virome datasets by MEGAN. Taxonomical annotation of contigs and unassembled reads with BLASTN and BLASTX with E value 10−3 to frozen versions of the NCBI databases nt and nr, respectively, applying the lowest common ancestor algorithm in MEGAN5 (Huson et al, 2007). (A) ALL BLASTN, (B) ALL BLASTX, (C) non-ALL BLASTN, (D) non-ALL BLASTX.
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