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. 2016 Aug 22:6:31715.
doi: 10.1038/srep31715.

Optical coherence microscopy in 1700 nm spectral band for high-resolution label-free deep-tissue imaging

Masahito Yamanaka  1 , Tatsuhiro Teranishi  1 , Hiroyuki Kawagoe  1 , Norihiko Nishizawa  1
Affiliations

Optical coherence microscopy in 1700 nm spectral band for high-resolution label-free deep-tissue imaging

Masahito Yamanaka et al. Sci Rep. .

Abstract

Optical coherence microscopy (OCM) is a label-free, high-resolution, three-dimensional (3D) imaging technique based on optical coherence tomography (OCT) and confocal microscopy. Here, we report that the 1700-nm spectral band has the great potential to improve the imaging depth in high-resolution OCM imaging of animal tissues. Recent studies to improve the imaging depth in OCT revealed that the 1700-nm spectral band is a promising choice for imaging turbid scattering tissues due to the low attenuation of light in the wavelength region. In this study, we developed high-resolution OCM by using a high-power supercontinuum source in the 1700-nm spectral band, and compared the attenuation of signal-to-noise ratio between the 1700-nm and 1300-nm OCM imaging of a mouse brain under the condition of the same sensitivity. The comparison clearly showed that the 1700-nm OCM provides larger imaging depth than the 1300-nm OCM. In this 1700-nm OCM, the lateral resolution of 1.3 μm and the axial resolution of 2.8 μm, when a refractive index was assumed to be 1.38, was achieved.

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Figures

Figure 1
Figure 1
(A) Optical setup of the optical coherence microscopy using the SC source in the 1700-nm spectral band and (B) optical spectrum of the SC source.
Figure 2
Figure 2
(A) Interference signal obtained with the developed OCM, (B) the logarithmically demodulated signal, (C) OCM image of a single polystyrene bead with a diameter of 200 nm in x-y plane (en-face) and the intensity profile spanning the white dotted line in the image, and (D) OCM image of a pig thyroid gland at a depth of 150 μm. (E) Sample observation configuration in this experiment. Scale bar: (C) 5 μm and (D) 50 μm.
Figure 3
Figure 3
Cross-sectional images of a mouse brain obtained with (A) the 1700-nm OCM and (B) the 1300-nm OCM. The lateral and axial width of the images were 100 and 1700 μm, respectively. (C) SNR of each OCM at difference depths. The dotted lines are exponential fits.
Figure 4
Figure 4
OCM images (x-y plane (en-face)) of (A) a pig thyroid grand and (B) a mouse brain at different imaging depths. Scale bar: 20 μm.
See this image and copyright information in PMC

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