Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Dec;52(6):883-886.
doi: 10.1007/s11262-016-1378-y. Epub 2016 Aug 17.

Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay

Yang Yang  1 , Xiaodong Qin  1 , Yingjun Sun  2 , Ting Chen  2 , Zhidong Zhang  3
Affiliations

Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay

Yang Yang et al. Virus Genes. 2016 Dec.

Abstract

A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was 70 copies of HP-PRRSV RNA/reaction. The real-time RT-RPA highly specific amplified HP-PRRSV with no cross-reaction with classic PRRSV, classic swine fever virus, pseudorabies virus, and foot-and-mouth disease virus. Assessment with 125 clinical samples showed that the developed real-time RT-RPA assay was well correlated with real-time RT-qPCR assays for detection of HP-PRRSV. These results suggest that the developed real-time RT-RPA assay is suitable for rapid detection of HP-PRRSV.

Keywords: HP-PRRSV; Molecular diagnosis; RT-qPCR; Real-time RT-RPA; Recombinase polymerase amplification.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Vet Microbiol. 1997 Apr;55(1-4):309-16 - PubMed
    1. Arch Virol. 1997;142(3):629-33 - PubMed
    1. PLoS Negl Trop Dis. 2014 Mar 06;8(3):e2730 - PubMed
    1. PLoS One. 2014 Aug 14;9(8):e104667 - PubMed
    1. J Clin Microbiol. 2012 Jul;50(7):2234-8 - PubMed

MeSH terms

LinkOut - more resources