Review
doi: 10.1042/BST20160079.
Structure determination of transient transcription complexes
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- PMID: 27528766
- DOI: 10.1042/BST20160079
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Review
Structure determination of transient transcription complexes
Biochem Soc Trans.
.
Abstract
The determination of detailed 3D structures of large and transient multicomponent complexes remains challenging. Here I describe the approaches that were used and developed by our laboratory to achieve structure solution of eukaryotic transcription complexes. I hope this collection serves as a resource for structural biologists seeking solutions for difficult structure determination projects.
Keywords: X-ray crystallography; cryo-electron microscopy; eukaryotic RNA polymerase complex; integrated structural biology; multiprotein assembly.
© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society.
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