Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Oct:2015:413-415.

High-Throughput Microfluidic Device for Circulating Tumor Cell Isolation from Whole Blood

Daniel K Yang  1 , Serena Leong  2 , Lydia L Sohn  1
Affiliations

High-Throughput Microfluidic Device for Circulating Tumor Cell Isolation from Whole Blood

Daniel K Yang et al. Micro Total Anal Syst. 2015 Oct.

Abstract

Circulating tumor cells (CTCs) are promising markers to determine cancer patient prognosis and track disease response to therapy. We present a multi-stage microfluidic device we have developed that utilizes inertial and Dean drag forces for isolating CTCs from whole blood. We demonstrate a 94.2% ± 2.1% recovery of cancer cells with our device when screening whole blood spiked with MCF-7 GFP cells.

Keywords: CTC; Inertial microfluidics; Size-based separation.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Schematic, isometric view, of our CTC isolation platform. A) Stage One consists of a symmetric CEA (sCEA) microfluidic channel in which cells are focused to the outer walls of the microchannel through a balance of wall lift and shear gradient lift forces. Stage Two consists of an asymmetric CEA (aCEA) microfluidic channel in which inertial focused streamlines are exposed to Dean drag forces where small particles migrate to different equilibrium positions. B) Overall percentage of MCF-7 GFP cells found in all collection and waste outlets. Data was obtain from processing 1 mL of whole blood spiked with MCF-7 GFP cells at a ratio of 10,000 WBCs: 1. Error bars correspond to standard deviation.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Hong B, et al. Detecting circulating tumor cells: current challenges and new trends. Theranostics. 2013;3:377–394. - PMC - PubMed
    1. Yu M, et al. Circulating tumor cells: approaches to isolation and characterization. The Journal of cell biology. 2011;192:373–382. - PMC - PubMed
    1. Phillips KG, et al. Optical quantification of cellular mass, volume, and density of circulating tumor cells identified in an ovarian cancer patient. Frontiers in oncology. 2012;2:72. - PMC - PubMed
    1. Park JM, et al. Highly efficient assay of circulating tumor cells by selective sedimentation with a density gradient medium and microfiltration from whole blood. Analytical chemistry. 2012;84:7400–7407. - PubMed
    1. Autebert J, et al. Microfluidic: an innovative tool for efficient cell sorting. Methods. 2012;57:297–307. - PubMed

LinkOut - more resources