Intracellular studies in the facial nucleus illustrating a simple new method for obtaining viable motoneurons in adult rat brain slices
- PMID: 2740992
- DOI: 10.1002/syn.890030406
Intracellular studies in the facial nucleus illustrating a simple new method for obtaining viable motoneurons in adult rat brain slices
Abstract
In general, it has been difficult to preserve electrophysiologically viable motoneurons in brain slices from adult mammals. The present study describes a new method for obtaining viable motoneurons in the facial nucleus of adult rat brain slices. The essence of the method was to use a modified artificial cerebrospinal fluid (ACSF) in which NaCl was replaced initially by sucrose; the modified ACSF was used during 1) preparation and 2) a 1 hr recovery period. The rationale for the modification is discussed in terms of the proposed acute neurotoxic effects of passive chloride entry and subsequent cell swelling and lysis. The actual recordings were made only after switching back to normal ACSF. Use of this method yielded large numbers of viable motoneurons that were suitable for intracellular recording; no motoneurons survived when normal ACSF (i.e., with NaCl) was used during slice preparation. A survey of some electrophysiological and pharmacological properties of facial motoneurons in this preparation, by means of current-clamp and voltage-clamp recording, revealed close similarities to the properties of adult motoneurons previously observed in vivo (e.g., time-dependent inward rectification, apamin-sensitive afterhyperpolarization, and serotonin-induced slow depolarization).
Similar articles
-
Patch-clamp studies in the CNS illustrate a simple new method for obtaining viable neurons in rat brain slices: glycerol replacement of NaCl protects CNS neurons.J Neurosci Methods. 2006 Dec 15;158(2):251-9. doi: 10.1016/j.jneumeth.2006.06.006. Epub 2006 Jul 13. J Neurosci Methods. 2006. PMID: 16842860
-
LSD has high efficacy relative to serotonin in enhancing the cationic current Ih: intracellular studies in rat facial motoneurons.Synapse. 1993 Feb;13(2):123-34. doi: 10.1002/syn.890130205. Synapse. 1993. PMID: 8446921
-
The vasopressin-induced excitation of hypoglossal and facial motoneurons in young rats is mediated by V1a but not V1b receptors, and is independent of intracellular calcium signalling.Eur J Neurosci. 2006 Sep;24(6):1565-74. doi: 10.1111/j.1460-9568.2006.05038.x. Eur J Neurosci. 2006. PMID: 17004920
-
Persistent sodium and calcium currents in rat hypoglossal motoneurons.J Neurophysiol. 2003 Jan;89(1):615-24. doi: 10.1152/jn.00241.2002. J Neurophysiol. 2003. PMID: 12522206
-
[Electrophysiologic characteristics of motor neurons of the cat facial nerve nucleus].Neirofiziologiia. 1978;10(3):261-70. Neirofiziologiia. 1978. PMID: 673073 Russian.
Cited by
-
Cortical feedback controls the frequency and synchrony of oscillations in the visual thalamus.J Neurosci. 2000 Oct 1;20(19):7478-88. doi: 10.1523/JNEUROSCI.20-19-07478.2000. J Neurosci. 2000. PMID: 11007907 Free PMC article.
-
Are the interlaminar zones of the ferret dorsal lateral geniculate nucleus actually part of the perigeniculate nucleus?J Neurosci. 1996 Oct 1;16(19):5923-41. doi: 10.1523/JNEUROSCI.16-19-05923.1996. J Neurosci. 1996. PMID: 8815875 Free PMC article.
-
Microglia Dynamics and Interactions with Motoneurons Axotomized After Nerve Injuries Revealed By Two-Photon Imaging.Sci Rep. 2020 May 26;10(1):8648. doi: 10.1038/s41598-020-65363-9. Sci Rep. 2020. PMID: 32457369 Free PMC article.
-
Ionic mechanisms mediating 5-hydroxytryptamine- and noradrenaline-evoked depolarization of adult rat facial motoneurones.J Physiol. 1992 Oct;456:473-90. doi: 10.1113/jphysiol.1992.sp019347. J Physiol. 1992. PMID: 1293283 Free PMC article.
-
Distinct receptors underlie glutamatergic signalling in inspiratory rhythm-generating networks and motor output pathways in neonatal rat.J Physiol. 2008 May 1;586(9):2357-70. doi: 10.1113/jphysiol.2007.150532. Epub 2008 Mar 13. J Physiol. 2008. PMID: 18339693 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources