Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jul 5;8(7):414.
doi: 10.3390/nu8070414.

Concord and Niagara Grape Juice and Their Phenolics Modify Intestinal Glucose Transport in a Coupled in Vitro Digestion/Caco-2 Human Intestinal Model

Sydney Moser  1 , Jongbin Lim  2 , Mohammad Chegeni  3 , JoLynne D Wightman  4 , Bruce R Hamaker  5   6 , Mario G Ferruzzi  5   6
Affiliations

Concord and Niagara Grape Juice and Their Phenolics Modify Intestinal Glucose Transport in a Coupled in Vitro Digestion/Caco-2 Human Intestinal Model

Sydney Moser et al. Nutrients. .

Abstract

While the potential of dietary phenolics to mitigate glycemic response has been proposed, the translation of these effects to phenolic rich foods such as 100% grape juice (GJ) remains unclear. Initial in vitro screening of GJ phenolic extracts from American grape varieties (V. labrusca; Niagara and Concord) suggested limited inhibitory capacity for amylase and α-glucosidase (6.2%-11.5% inhibition; p < 0.05). Separately, all GJ extracts (10-100 µM total phenolics) did reduce intestinal trans-epithelial transport of deuterated glucose (d7-glu) and fructose (d7-fru) by Caco-2 monolayers in a dose-dependent fashion, with 60 min d7-glu/d7-fru transport reduced 10%-38% by GJ extracts compared to control. To expand on these findings by assessing the ability of 100% GJ to modify starch digestion and glucose transport from a model starch-rich meal, 100% Niagara and Concord GJ samples were combined with a starch rich model meal (1:1 and 1:2 wt:wt) and glucose release and transport were assessed in a coupled in vitro digestion/Caco-2 cell model. Digestive release of glucose from the starch model meal was decreased when digested in the presence of GJs (5.9%-15% relative to sugar matched control). Furthermore, transport of d7-glu was reduced 10%-38% by digesta containing bioaccessible phenolics from Concord and Niagara GJ compared to control. These data suggest that phenolics present in 100% GJ may alter absorption of monosaccharides naturally present in 100% GJ and may potentially alter glycemic response if consumed with a starch rich meal.

Keywords: anthocyanins; carbohydrate digestion; glucose transport; grape juice.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Impact of 2013 and 2014 harvest grape juice extracts on d7-fructose (A,B) or d7-glucose (C,D) transport across Caco-2 human intestinal cell monolayer. Data is represented as concentration of deuterated sugar in basolateral compartment at 60 min. Data represent mean ± SEM for n = 4 replicate wells at each time point. Presence of different letters between values indicates significant differences in glucose transport between treatments within each concentration (p < 0.05).
Figure 2
Figure 2
Impact of 2013 and 2014 100% grape juice aqueous digesta (AQ) on d7-glucose transport across Caco-2 human intestinal cell monolayers over 60 min. Data represent mean ± SEM for n = 4 replicate wells. * indicates significant difference in basolateral glucose concentration (mM) compared to control (p < 0.05).
Figure 3
Figure 3
d7-Glucose transport across Caco-2 human intestinal cell monolayers from AQ digesta of co-digested GJ and starch rich test meal. Data is represented as a concentration of deuterated glucose in basolateral compartment at 60 min by treatment compared to control over 60 min. Data represent mean ± SEM for n = 4 replicate wells at each time point. Presence of different letter between values indicates significant differences in d7-glucose transport between treatments within the same ratio of beverage to meal.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. United States of Department of Agriculture (USDA) Fruit Yearbook Supply and Utilization. USDA; Washington, DC, USA: 2015.
    1. Dani C., Oliboni L.S., Vanderlinde R., Bonatto D., Salvador M., Henriques J.A.P. Phenolic content and antioxidant activities of white and purple juices manufactured with organically- or conventionally-produced grapes. Food Chem. Toxicol. 2007;45:2574–2580. - PubMed
    1. Fuleki T., Ricardo-Da-Silva J.M. Effects of cultivar and processing method on the contents of catechins and procyanidins in grape juice. J. Agric. Food Chem. 2003;51:640–646. doi: 10.1021/jf020689m. - DOI - PubMed
    1. Iyer M.M., Sacks G.L., Padilla-Zakour O.I. Impact of harvesting and processing conditions on green leaf volatile development and phenolics in Concord grape juice. J. Food Sci. 2010;75:297–304. doi: 10.1111/j.1750-3841.2010.01559.x. - DOI - PubMed
    1. Stalmach A., Edwards C.A., Wightman J.D., Crozier A. Identification of (poly) phenolic compounds in concord grape juice and their metabolites in human plasma and urine after juice consumption. J. Agric. Food Chem. 2011;59:9512–9522. doi: 10.1021/jf2015039. - DOI - PubMed

MeSH terms

LinkOut - more resources