Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jun 14:7:228.
doi: 10.3389/fphys.2016.00228. eCollection 2016.

Understanding the Cap Structure in K2P Channels

Leandro Zúñiga  1 , Rafael Zúñiga  1
Affiliations

Understanding the Cap Structure in K2P Channels

Leandro Zúñiga et al. Front Physiol. .
No abstract available

Keywords: K2P channels; K2P potassium channels structure; cap structure; potassium channels; two-pore domain channel.

PubMed Disclaimer

Figures

Figure 1
Figure 1
K2P channels: (A) Phylogenetic tree for K2P family (the phylogenic analysis was done with MEGA software version 5). The length of lines indicates the relative distances between nodes and it is scaled below the tree. Numbers on branches indicate bootstrap values (as a percentage). (B) Topological model proposed for K2P channels, each subunit has two pore forming domain (P loops) and four transmembrane domains (denoted M1-M4). (C) Amino acid sequence alignment of K2P channel M1-P1 domain. Gaps are indicated by dashes, letters with black background are identical amino acids, and letters with gray background are similar amino acids. Red arrow indicates the cysteine residues involved in the formation of covalent bridge between two subunits. Amino acid identity (ID) among the M1-P1 domains of hTWIK-1 and the others K2P subunits was calculated. (D) View of the TRAAK structure (PDB code 3UM7) in ribbon representation, showing a non–swapped configuration (conventional configuration). (E) View of the TRAAK structure (PDB code 4I9W) in a domain swapped configuration. The potassium ions are colored orange and the chains are in blue and yellow. The disulfide bridge at the top of the cap is shown in stick representation colored cyan. (F) View from the extracellular side showing the TRAAK pore (PDB code 3UM7). The GYG sequence of each P domain is highlighted in stick representation and the helical cap was removed. For clarity in (D,E), the second pore domain was removed from each TRAAK subunits.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Barel O., Shalev S. A., Ofir R., Cohen A., Zlotogora J., Shorer Z., et al. . (2008). Maternally inherited Birk Barel mental retardation dysmorphism syndrome caused by a mutation in the genomically imprinted potassium channel KCNK9. Am. J. Hum. Genet. 83, 193–199. 10.1016/j.ajhg.2008.07.010 - DOI - PMC - PubMed
    1. Berg A. P., Talley E. M., Manger J. P., Bayliss D. A. (2004). Motoneurons express heteromeric TWIK-related acid-sensitive K+ (TASK) channels containing TASK-1 (KCNK3) and TASK-3 (KCNK9) subunits. J. Neurosci. 24, 6693–6702. 10.1523/JNEUROSCI.1408-04.2004 - DOI - PMC - PubMed
    1. Blin S., Ben S., Kim E. J., Brau F., Kang D., Bichet D., et al. (2016). Mixing and matching TREK/TRAAK subunits generate heterodimeric K2P channels with unique properties. Proc. Natl. Acad. Sci. U.S.A 113, 4200–4205. 10.1073/pnas.1522748113 - DOI - PMC - PubMed
    1. Blin S., Chatelain F. C., Feliciangeli S., Kang D., Lesage F., Bichet D. (2014). Tandem pore domain halothane-inhibited K+ channel subunits THIK1 and THIK2 assemble and form active channels. J. Biol. Chem. 289, 28202–28212. 10.1074/jbc.M114.600437 - DOI - PMC - PubMed
    1. Brohawn S. G., Campbell E. B., MacKinnon R. (2013). Domain-swapped chain connectivity and gated membrane access in a Fab-mediated crystal of the human TRAAK K+ channel. Proc. Natl. Acad. Sci. U.S.A 110, 2129–2134. 10.1073/pnas.1218950110 - DOI - PMC - PubMed

LinkOut - more resources