Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jun 24:13:110.
doi: 10.1186/s12985-016-0569-4.

Alphacoronavirus in urban Molossidae and Phyllostomidae bats, Brazil

Affiliations

Alphacoronavirus in urban Molossidae and Phyllostomidae bats, Brazil

Karen Miyuki Asano et al. Virol J. .

Erratum in

Abstract

Background: Bats have been implicated as the main reservoir of coronavirus (CoV). Thus the role of these hosts on the evolution and spread of CoVs currently deserve the attention of emerging diseases surveillance programs. On the view of the interest on and importance of CoVs in bats the occurrence and molecular characterization of CoV were conducted in bats from Brazil.

Findings: Three hundred five enteric contents of 29 bat species were tested using a panCoV nested RT-PCR. Nine specimens were positive and eight was suitable for RdRp gene sequencing. RdRp gene phylogeny showed that all CoVs strains from this study cluster in Alphacoronavirus genus, with one Molossidae and one Phlyllostomidae-CoV specific groups. Phylogenetic analyses of two S gene sequences showed a large diversity within the Alphacoronavirus genus.

Conclusions: This study indicated a CoV-to-host specificity and draws attention for CoV detection in Cynomops sp, a potential new reservoir. The phylogenetic analyses indicate that diversity of CoV in bats is higher than previously known.

Keywords: Bat; Coronavirus; Molossidae; Phyllostomidae.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
phylogenetic tree constructed with neighbor-joining method and maximum likelihood composite substitution model for partial 393 bp fragment of coronavirus RdRp gene. Numbers on each node represents the bootstrap values. The scale represents the number of substitutions sites. Samples of this study are identified with a black triangle
Fig. 2
Fig. 2
phylogenetic tree constructed with the neighbor-joining method and maximum likelihood composite substitution model for partial 547 bp fragment of coronavirus S gene. Numbers on each node represents the bootstrap values. The scale represents the number of substitutions sites. Samples of this study are identified with a black triangle

Similar articles

Cited by

References

    1. Li W, Shi Z, Yu M, Ren W, Smith C, Epstein JH, et al. Bats are natural reservoirs of SARS-like coronaviruses. Science. 2008;310:676–9. doi: 10.1126/science.1118391. - DOI - PubMed
    1. Wang Q, Qi J, Yuan Y, Xuan Y, Han P, Wan Y, et al. Bat origins of MERS-CoV supported by bat coronavirus HKU4 usage of human receptor CD26. Cell Host Microbe. 2014;16:328–37. doi: 10.1016/j.chom.2014.08.009. - DOI - PMC - PubMed
    1. De Groot RJ, Baker SC, Baric R, Enjuanes L, Gorbalenya AE, Holmes KV, et al. Family coronaviridae. In: King A, Adams M, Cartens E, Lefkowitz E, et al., editors. Virus taxonomy: classification and nomenclature of viruses: ninth report of the International committee on taxonomy of viruses. San Diego, CA: Elsevier Academic Press; 2012. pp. 806–20.
    1. Woo PCY, Lau SK, Lam CS, Lai KK, Huang Y, Lee P, et al. Comparative analysis of complete genome sequences of three avian coronaviruses reveals a novel group 3c coronavirus. J Virol. 2009;83:908–17. doi: 10.1128/JVI.01977-08. - DOI - PMC - PubMed
    1. Woo PCY, Lau SK, Lam CS, Lau CC, Tsang AK, Lau JH, et al. Discovery of seven novel Mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus. J Virol. 2012;86:3995–4008. doi: 10.1128/JVI.06540-11. - DOI - PMC - PubMed

Publication types

LinkOut - more resources