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. 2016:1440:139-50.
doi: 10.1007/978-1-4939-3676-2_11.

Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus

Vanina Dengler  1 , Nadine McCallum  2
Affiliations

Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus

Vanina Dengler et al. Methods Mol Biol. 2016.

Abstract

Luciferase reporter gene fusions provide an extremely rapid and sensitive tool for measuring the induction or repression of stress responses in bacteria. Staphylococcus aureus activates the expression of a cell wall stress stimulon (CWSS) in response to the inhibition or disruption of cell wall synthesis. The highly sensitive promoter-reporter gene fusion construct psas016 p-luc+ can be used to quantify and compare any changes in CWSS expression levels and induction kinetics. Potential uses of this system include identifying and characterizing novel cell wall-targeting antibacterial agents, identifying genomic loci influencing cell envelope synthesis and detecting changes in CWSS expression that could be linked to decreased antibiotic susceptibility profiles in clinical isolates.

Keywords: Cell wall stress; Cell wall stress stimulon; Luciferase reporter gene fusion; Staphylococcus aureus; VraTSR.

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