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. 2018 Jul;24(7):494-501.
doi: 10.1007/s11655-016-2262-2. Epub 2016 Jun 9.

Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro

Qi-Qi Xin  1   2   3 , Bin-Rui Yang  2 , He-Feng Zhou  2 , Yan Wang  1   3 , Bo-Wen Yi  4 , Wei-Hong Cong  3 , Simon Ming-Yuen Lee  2 , Ke-Ji Chen  5
Affiliations

Paeoniflorin Promotes Angiogenesis in A Vascular Insufficiency Model of Zebrafish in vivo and in Human Umbilical Vein Endothelial Cells in vitro

Qi-Qi Xin et al. Chin J Integr Med. 2018 Jul.

Abstract

Objective: To investigate the pro-angiogenic effects of paeoniflorin (PF) in a vascular insufficiency model of zebrafish and in human umbilical vein endothelial cells (HUVECs).

Methods: In vivo, the pro-angiogenic effects of PF were tested in a vascular insufficiency model in the Tg(fli-1:EGFP)y1 transgenic zebrafish. The 24 h post fertilization (hpf) embryos were pretreated with vascular endothelial growth factor (VEGF) receptor tyrosine kinase inhibitor II (VRI) for 3 h to establish the vascular insufficiency model and then post-treated with PF for 24 h. The formation of intersegmental vessels (ISVs) was observed with a fluorescence microscope. The mRNA expression of fms-like tyrosine kinase-1 (flt-1), kinase insert domain receptor (kdr), kinase insert domain receptor like (kdrl) and von Willebrand factor (vWF) were analyzed by real-time polymerase chain reaction (PCR). In vitro, the pro-angiogenic effects of PF were observed in HUVECs in which cell proliferation, migration and tube formation were assessed.

Results: PF (6.25-100 μmol/L) could rescue VRI-induced blood vessel loss in zebrafish and PF (25-100 μmol/L), thereby restoring the mRNA expressions of flt-1, kdr, kdrl and vWF, which were down-regulated by VRI treatment. In addition, PF (0.001-0.03 μmol/L) could promote the proliferation of HUVECs while PF stimulated HUVECs migration at 1.0-10 μmol/L and tube formation at 0.3 μmol/L.

Conclusion: PF could promote angiogenesis in a vascular insufficiency model of zebrafish in vivo and in HUVECs in vitro.

Keywords: angiogenesis; human umbilical vein endothelial cell; paeoniflorin; zebrafish.

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References

    1. Toxicol In Vitro. 2012 Apr;26(3):455-61 - PubMed
    1. Stroke. 1994 Sep;25(9):1794-8 - PubMed
    1. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005 Apr;25(4):345-9 - PubMed
    1. Chin J Integr Med. 2015 Aug 14;: - PubMed
    1. Cell Adh Migr. 2009 Apr-Jun;3(2):216-23 - PubMed

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