Characterization of nucleocytoplasmic shuttling and intracellular localization signals in Duck Enteritis Virus UL54

doi:10.1016/j.biochi.2016.05.003

. 2016 Aug:127:86-94.

doi: 10.1016/j.biochi.2016.05.003. Epub 2016 May 5.

Characterization of nucleocytoplasmic shuttling and intracellular localization signals in Duck Enteritis Virus UL54

Chaoyue Liu¹, Anchun Cheng², Mingshu Wang³, Shun Chen¹, Renyong Jia¹, Dekang Zhu⁴, Mafeng Liu¹, Kunfeng Sun¹, Qiao Yang¹, Xiaoyue Chen⁴

Affiliations

¹ Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China.
² Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China. Electronic address: chenganchun@vip.163.com.
³ Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China. Electronic address: mshwang@163.com.
⁴ Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China.

PMID: 27157269
DOI: 10.1016/j.biochi.2016.05.003

Characterization of nucleocytoplasmic shuttling and intracellular localization signals in Duck Enteritis Virus UL54

Chaoyue Liu et al. Biochimie. 2016 Aug.

. 2016 Aug:127:86-94.

doi: 10.1016/j.biochi.2016.05.003. Epub 2016 May 5.

Authors

Chaoyue Liu¹, Anchun Cheng², Mingshu Wang³, Shun Chen¹, Renyong Jia¹, Dekang Zhu⁴, Mafeng Liu¹, Kunfeng Sun¹, Qiao Yang¹, Xiaoyue Chen⁴

Affiliations

¹ Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China.
² Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China. Electronic address: chenganchun@vip.163.com.
³ Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China. Electronic address: mshwang@163.com.
⁴ Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Wenjiang, Chengdu City, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China.

PMID: 27157269
DOI: 10.1016/j.biochi.2016.05.003

Abstract

Duck Enteritis virus (DEV) UL54 is a homolog of herpes simplex virus-1 (HSV-1) trafficking protein ICP27, which plays an essential role in infection. In this study, DEV UL54 shuttling between the nucleus and cytoplasm was verified with a heterokaryon assay. One predicted nuclear export sequence (NES) (339-348 aa) was shown to be functional and chromosomal region maintenance 1 (CRM1)-dependent; however, the insensitivity of UL54 to Leptomycin B (LMB) and NES mutation suggests that other mechanisms are responsible for the observed nuclear export. Next, three non-classical nuclear localization sequences (NLSs), referred to as NLS1 (105-122 aa), NLS2 (169-192 aa) and NLS3 (257-274 aa), were identified. Furthermore, a recombinant DEV with the UL54 NLSs deleted (DEV- UL54 mNLSs) was constructed and showed that UL54 NLSs moderately affected DEV growth.

Keywords: DEV; Nuclear export signal; Nuclear localization signal; Shuttling; UL54.

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Characterization of nucleocytoplasmic shuttling and intracellular localization signals in Duck Enteritis Virus UL54

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Characterization of nucleocytoplasmic shuttling and intracellular localization signals in Duck Enteritis Virus UL54

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