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. 2016 Mar-Apr;20(2):151-6.
doi: 10.4103/0972-124X.176390.

Fibrin network pattern changes of platelet-rich fibrin in young versus old age group of individuals: A cell block cytology study

Shravanthi Raghav Yajamanya  1 , Anirban Chatterjee  1 , Chaitanya Nischay Babu  2 , Deepika Karunanithi  2
Affiliations

Fibrin network pattern changes of platelet-rich fibrin in young versus old age group of individuals: A cell block cytology study

Shravanthi Raghav Yajamanya et al. J Indian Soc Periodontol. 2016 Mar-Apr.

Abstract

Background: To evaluate variations in fibrin network patterns of the platelet-rich fibrin (PRF) in different age groups.

Materials and methods: Ninety-five patients were divided into three age groups: Group 1: (20-39 years); Group 2: (40-59 years); and Group 3: (60 years and above). PRF was prepared from blood samples of all patients and were subjected to cell block cytology method of histological analysis and slides were prepared to histologically assess the age-related changes in (i) fibrin network patterns in terms of density and (ii) entrapment of platelets and white blood cells (WBCs) within fibrin meshwork.

Results: Two types of fibrin network pattern arrangements noticed: Dense and loose types in three age groups. However, there was a noticeable decrease in the dense type of fibrin network with progressing age and increase in the loose type of fibrin arrangement. Furthermore, variation in a number of platelets and WBCs entrapped within fibrin network in relation to age was noticed.

Conclusion: From the current study it can be concluded that age can be considered as one of the influencing factors on quality of PRF in terms of fibrin network patterns and hence, platelet and WBCs entrapment within these fibrin networks.

Keywords: Age; fibrin network patterns; platelet-rich fibrin; platelets; white blood cell.

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Figures

Figure 1
Figure 1
Platelet-rich fibrin preparation (a) blood collection; (b) centrifugation machine; (c) platelet-rich fibrin clot; (d) platelet-rich fibrin clot with red blood cell's layer
Figure 2
Figure 2
Platelet-rich fibrin clot slide preparation for histological analysis (a) fixing and labeling of platelet-rich fibrin clot; (b) tissue processing (c) embedding; (d) prepared slide
Figure 3
Figure 3
Histologic presentation of fibrin network pattern in platelet rich fibrin clot in age groups: (a) Age group 20 years: Blue areas – platelet and white blood cell aggregates, pink areas – dense fibrin network predominantly, packeting like pattern and (b) age group 30 years: Red areas – red blood cell, blue areas – platelets and white blood cell aggregates, dark pink areas – dense fibrin network pattern, light pink areas – loose fibrin network pattern
Figure 4
Figure 4
Histologic presentation of fibrin network pattern in platelet rich fibrin clot in the age group of the late 30s and 40 years. Red areas – red blood cells, blue areas – platelets and white blood cell (layered arrangement), dark pink – dense fibrin network with interspersed loose fibrin network, light pink – loose fibrin network
Figure 5
Figure 5
Histologic presentation of fibrin network pattern in platelet rich fibrin clot in (a) age group 50 years: Red area – red blood cells, blue area – platelets and white blood cells, dark pink area – dense fibrin network, light pink area – loose fibrin network (scanty) and (b) age group 60 and above: Red area – red blood cells, blue area – platelets and white blood cells, blue area – platelets and white blood cells, dark pink area – dense fibrin network, light pink area – loose fibrin network
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