Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 May;13(5):4167-74.
doi: 10.3892/mmr.2016.5070. Epub 2016 Mar 30.

Extracts of Bauhinia championii (Benth.) Benth. attenuate the inflammatory response in a rat model of collagen-induced arthritis

Wei Xu  1 , Mingqing Huang  1 , Yuqin Zhang  1 , Huang Li  1 , Haiyin Zheng  2 , Lishuang Yu  1 , Kedan Chu  1 , Yu Lin  1 , Lidian Chen  3
Affiliations

Extracts of Bauhinia championii (Benth.) Benth. attenuate the inflammatory response in a rat model of collagen-induced arthritis

Wei Xu et al. Mol Med Rep. 2016 May.

Abstract

Rheumatoid arthritis is considered a serious public health problem, which is commonly treated with traditional Chinese or herbal medicine. The present study evaluated the effects of Bauhinia championii (Benth.) Benth. extraction (BCBE) on a type II collagen-induced arthritis (CIA) rat model. Wistar rats with CIA received either 125 or 500 mg/kg BCBE, after which, paw swelling was markedly suppressed compared with in the model group. In addition, BCBE significantly ameliorated pathological joint alterations, including synovial hyperplasia, and cartilage and bone destruction. The protein and mRNA expression levels of interleukin (IL)‑6, IL‑8, tumor necrosis factor‑α and nuclear factor‑κB in synovial tissue were determined by immunohistochemical staining, western blot analysis and reverse transcription‑polymerase chain reaction. The results demonstrated that the expression levels of these factors were significantly downregulated in the BCBE‑treated group compared with in the model group. These results indicated that BCBE may exert an inhibitory effect on the CIA rat model, and its therapeutic potential is associated with its anti-inflammatory action.

PubMed Disclaimer

Figures

Figure 1
Figure 1
High-performance liquid chromatography profiles of (A) control solution and (B) Bauhinia championii (Benth.) Benth. extraction. Peak 1, myricetin; peak 2, quercetin; peak 3, kaempferol.
Figure 2
Figure 2
Right hind paw edema in the various rat groups. (A) Morphological characteristics of the right hind paw from the various rat groups. (B) Paw edema volume of the various rat groups. ##P<0.01 vs. the control group; *P<0.05, **P<0.01 vs. the model group. Data are presented as the mean ± standard deviation. BCBE, Bauhinia championii (Benth.) Benth. extraction; TGT, tripterygium glycosides tablet.
Figure 3
Figure 3
Effects of Bauhinia championii (Benth.) Benth. extraction (BCBE) on the histopathology of rat ankle joints. Representative images are presented (hematoxylin and eosin; magnification, 4×). TGT, tripterygium glycosides tablet.
Figure 4
Figure 4
Effects of Bauhinia championii (Benth.) Benth. extraction (BCBE) on the mRNA expression levels of interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-α and nuclear factor (NF)-κB. The levels of IL-6, IL-8, TNF-α and NF-κB were determined in synovial tissues using reverse transcription-polymerase chain reaction. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. TGT, tripterygium glycosides tablet.
Figure 5
Figure 5
Effects of Bauhinia championii (Benth.) Benth. extraction (BCBE) on the expression of interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-α and nuclear factor (NF)-κB. The levels of (A) IL-6, (B) IL-8 and (C) TNF-α in synovial tissues were determined by immunohistochemistry (magnification ×40), and (D) the levels of NF-κB in synovial tissues were determined by western blotting. β-actin was used as a control. TGT, tripterygium glycosides tablet.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Brenner M, Meng HC, Yarlett NC, Joe B, Griffiths MM, Remmers EF, Wilder RL, Gulko PS. The non-MHC quantitative trait locus Cia5 contains three major arthritis genes that differentially regulate disease severity, pannus formation, and joint damage in collagen- and pristane-induced arthritis. J Immunol. 2005;174:7894–7903. doi: 10.4049/jimmunol.174.12.7894. - DOI - PubMed
    1. Ono Y, Inoue M, Mizukami H, Ogihara Y. Suppressive effect of Kanzo-bushi-to, a Kampo medicine, on collagen-induced arthritis. Biol Pharm Bull. 2004;27:1406–1413. doi: 10.1248/bpb.27.1406. - DOI - PubMed
    1. Helliwell P, Woodburn J, Redmond A, Turner D, Davys H, editors. The Foot and Ankle in Rheumatoid Arthritis: A Comprehensive Guide. Elsevier Health Sciences; Edinburgh: 2007. Current concepts in rheumatoid arthritis; pp. 1–16.
    1. Kochi Y, Suzuki A, Yamada R, Yamamoto K. Genetics of rheumatoid arthritis: Underlying evidence of ethnic differences. J Autoimmun. 2009;32:158–162. doi: 10.1016/j.jaut.2009.02.020. - DOI - PubMed
    1. Symmons DP. Environmental factors and the outcome of rheumatoid arthritis. Best Pract Res Clin Rheumatol. 2003;17:717–727. doi: 10.1016/S1521-6942(03)00063-9. - DOI - PubMed