Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jan 19:11:315-24.
doi: 10.2147/IJN.S93611. eCollection 2016.

Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment

Rafał Banasiuk  1 , Joanna E Frackowiak  2 , Marta Krychowiak  1 , Marta Matuszewska  1 , Anna Kawiak  1 , Magdalena Ziabka  3 , Zofia Lendzion-Bielun  4 , Magdalena Narajczyk  5 , Aleksandra Krolicka  1
Affiliations

Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment

Rafał Banasiuk et al. Int J Nanomedicine. .

Abstract

A fast, economical, and reproducible method for nanoparticle synthesis has been developed in our laboratory. The reaction is performed in an aqueous environment and utilizes light emitted by commercially available 1 W light-emitting diodes (λ =420 nm) as the catalyst. This method does not require nanoparticle seeds or toxic chemicals. The irradiation process is carried out for a period of up to 10 minutes, significantly reducing the time required for synthesis as well as environmental impact. By modulating various reaction parameters silver nanoparticles were obtained, which were predominantly either spherical or cubic. The produced nanoparticles demonstrated strong antimicrobial activity toward the examined bacterial strains. Additionally, testing the effect of silver nanoparticles on the human keratinocyte cell line and human peripheral blood mononuclear cells revealed that their cytotoxicity may be limited by modulating the employed concentrations of nanoparticles.

Keywords: antimicrobial activity; green synthesis; nanocubes; nanospheres.

PubMed Disclaimer

Figures

Figure 1
Figure 1
TEM and SEM images of silver nanoparticles synthesized using (A) TEM, (B) SEM glycine, (C) TEM, (D) SEM PVP, (E) TEM, (F) SEM SDS. Abbreviations: PVP, polyvinylpyrrolidone; SEM, scanning electron microscopy; SDS, sodium dodecyl sulfate; TEM, transmission electron microscopy.
Figure 2
Figure 2
TEM images of silver nanoparticles synthesized using 1 mM AgNO3, 1 mL 5% NaClO per 100 mL and (A) 400 mg PVP per 100 mL, (B) 200 mg PVP per 100 mL; irradiation time 10 minutes, light wavelength 420 nm. Abbreviations: PVP, polyvinylpyrrolidone; TEM, transmission electron microscopy.
Figure 3
Figure 3
TEM images of silver nanoparticles synthesized using 2 mM AgNO3, 5 mM SDS, 0.5 mL 5% NaClO per 100 mL; irradiation time 10 minutes, light wavelength 420 nm. Abbreviations: SDS, sodium dodecyl sulfate; TEM, transmission electron microscopy.
Figure 4
Figure 4
TEM images of Pseudomonas aeruginosa PAK: (A) control, (B) after 9 hours incubation with 2 MIC PVP-capped nanoparticles. Abbreviations: 2 MIC, double minimal inhibitory concentration; PVP, polyvinylpyrrolidone; TEM, transmission electron microscopy.
Figure 5
Figure 5
Cytotoxic effect of various concentrations of AgNPs on human keratinocytes (n=3). Notes: Data were analyzed by one-way ANOVA with Tukey’s post hoc tests; P≤0.05 (*) indicates differences between the control and one-treated cells. Toxicity was assessed after 24 hours of incubation at 37°C in the dark. Abbreviations: AgNPs, silver nanoparticles; ANOVA, analysis of variance.
Figure 6
Figure 6
Cytotoxic effects of AgNPs on human PBMCs. Notes: Untreated control (A) and PBMCs treated with 0.2 μg/mL of AgNPs (B) consist of two distinct populations (lymphocytes and monocytes), which predominantly are Annexin V and 7-AAD negative, indicating their viability. PBMCs treated with AgNPs at a concentration of 0.5 μg/mL (C) and higher (2 μg/mL [D]) demonstrate a change in their morphology as smaller and more granular cells are observed, expressed in FSC and SSC. Changes also include their membrane, resulting in either an Annexin V-positive or both Annexin V and 7-AAD-positive staining for the majority of cells, demonstrating a high death percentage. The cytometric analysis shows two representative experiments. Abbreviations: 7-AAD, 7-aminoactinomycin D; AgNPs, silver nanoparticles; FSC, forward scatter; PBMCs, peripheral blood mononuclear cells; SSC, side scatter.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Kim J, Kuk E, Yu K, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007;3:95–101. - PubMed
    1. Rai M, Deshmukh S, Ingle A, Gade A. A silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria. J Appl Microbiol. 2012;112:841–852. - PubMed
    1. Yin Y, Liu J, Jiang G. Sunlight-induced reduction of ionic Ag and Au to metallic nanoparticles by dissolved organic matter. ACS Nano. 2012;6:7910–7919. - PubMed
    1. Wiley B, Herricks T, Sun Y, Xia Y. Polyol synthesis of silver nanoparticles: use of chloride and oxygen to promote the formation of single-crystal, truncated cubes and tetrahedrons. Nano Lett. 2004;4:1733–1739.
    1. Byeon J, Kim Y. A novel polyol method to synthesize colloidal silver nanoparticles by ultrasonic irradiation. Ultrasonics Sonochem. 2012;19:209–215. - PubMed

Publication types

MeSH terms

LinkOut - more resources