A new Saccharomyces cerevisiae mnn mutant N-linked oligosaccharide structure
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A new Saccharomyces cerevisiae mnn mutant N-linked oligosaccharide structure
J Biol Chem.
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Abstract
We find that the N-linked Man8GlcNAc2- core oligosaccharide of Saccharomyces cerevisiae mnn mutant mannoproteins is enlarged by the addition of the outer chain to the alpha 1----3-linked mannose in the side chain that is attached to the beta 1----4-linked mannose rather than by addition to the terminal alpha 1----6-linked mannose. This conclusion is derived from structural studies on a phosphorylated oligosaccharide fraction and from mass spectral fragment analysis of neutral core oligosaccharides.
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