doi: 10.1007/s13361-015-1299-3.
Epub 2015 Nov 30.
Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides
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- PMID: 26620529
- DOI: 10.1007/s13361-015-1299-3
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Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides
J Am Soc Mass Spectrom.
2016 Feb.
Free article
Abstract
This is the first study where graphene is used as a MALDI adjuvant in combination with the traditional matrix α-cyano-4-hydroxycinnamic acid (CHCA) to improve the signal intensity of peptide samples. Use of this amended matrix not only leads to increased signals but also to a higher number of peaks detected in complex samples. Additionally, the use of graphene has a stabilizing effect that can also be exploited to improve the detection of easily cleavable molecules. Graphical Abstract ᅟ.
Keywords: Graphene; MALDI-TOF/TOF; Phosphorylated peptides.
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