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. 2015 Dec 15;25(24):5737-42.
doi: 10.1016/j.bmcl.2015.10.078. Epub 2015 Oct 26.

Development of surface immobilized 3-azidocoumarin-based fluorogenic probe via strain promoted click chemistry

M Vijaya Bharathi  1 , Mohit Chhabra  1 , Priyankar Paira  2
Affiliations

Development of surface immobilized 3-azidocoumarin-based fluorogenic probe via strain promoted click chemistry

M Vijaya Bharathi et al. Bioorg Med Chem Lett. .

Abstract

A new class of imaging probe, a fluorogenic version of 1, 3-dipolar cycloaddition of azides and alkynes has been developed. 3-azidocoumarin scaffolds were selectively immobilized on the DBCO modified bead surface via SPAAC and provide direct and strong fluorescence in fluorescence microscopy. This developed click-on beads could be applied to label various biomolecules, such as nucleic acids, proteins and other molecules. To this end, 5'(7-hydroxy 3-azido coumarin) labelled DNA primer also displayed strong fluorescence upon successful immobilization on the bead surface.

Keywords: 3-Azido coumarin; Click chemistry; Fluorogenic probe; Polystyrene beads; SPAAC.

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