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. 2015 Sep 8;8(9):e22295.
doi: 10.5812/jjm.22295. eCollection 2015 Sep.

Phenotypic and Genotypic Characterization of Enteroaggregative Escherichia coli Strains Isolated From Diarrheic Children in Iran

Abolfazl Davoodabadi  1 , Maryam Abbaszadeh  2 , Mana Oloomi  2 , Saeid Bouzari  2
Affiliations

Phenotypic and Genotypic Characterization of Enteroaggregative Escherichia coli Strains Isolated From Diarrheic Children in Iran

Abolfazl Davoodabadi et al. Jundishapur J Microbiol. .

Abstract

Background: Several studies performed in developed and developing countries have identified enteroaggregative Escherichia coli (EAEC) as the emerging cause of pediatric diarrhea.

Objectives: This study investigated the phenotypic and genetic characteristics of EAEC strains isolated from children with diarrhea between 2007 - 2008 in Tehran, Iran.

Materials and methods: EAEC strains were examined for virulence plasmid genes (aap, aggR, and aatA), biofilm formation, and drug resistance. In addition, pulsed-field gel electrophoresis (PFGE) profiles of these strains were determined.

Results: Significant percentage of local EAEC strains carried the virulence plasmid genes and formed biofilms. In addition, these strains showed high resistance to ampicillin (100%), tetracycline (65.7%), streptomycin (58.7%), chloramphenicol (52.6%), and trimethoprim/sulfamethoxazole (51.7%) and had different PFGE patterns.

Conclusions: These results indicated that EAEC strains isolated from Iranian children with diarrhea were heterogeneous and showed high resistance rates against commonly used antibiotics, which was similar to that reported in studies performed in other countries.

Keywords: Biofilms; Drug Resistance; Electrophoresis Gel Pulsed-Field; Escherichia coli; Virulence Factors.

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Figures

Figure 1.
Figure 1.. Multiplex PCR for the 3 Plasmid Genes aap (310 bp), aggR (457 bp), and aatA
Lane M, 50-bp DNA marker; lane 1, EAEC strain 042 as the positive control; lane 2, E. coli K12 as the negative control; lane 3 - 9, EAEC strains.
Figure 2.
Figure 2.. Dendrogram Generated Using the GelCompar Software Showing the Distance Calculated using the Dice Similarity Index of PFGE XbaI Profiles of 31 EAEC Strains
Strains with a similarity of ≥ 80% were included in a single clonal group. Antibiotics: AM, Ampicillin, C, Chloramphenicol; GM, Gentamicin; S, Streptomycin; TE, Tetracycline; K, Kanamycin; CF, Cephalothin; CT, ceftizoxime; SXT, trimethoprim/sulfamethoxazole.
See this image and copyright information in PMC

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References

    1. Boisen N, Scheutz F, Rasko DA, Redman JC, Persson S, Simon J, et al. Genomic characterization of enteroaggregative Escherichia coli from children in Mali. J Infect Dis. 2012;205(3):431–44. doi: 10.1093/infdis/jir757. - DOI - PMC - PubMed
    1. Huang DB, Mohanty A, DuPont HL, Okhuysen PC, Chiang T. A review of an emerging enteric pathogen: enteroaggregative Escherichia coli. J Med Microbiol. 2006;55(Pt 10):1303–11. doi: 10.1099/jmm.0.46674-0. - DOI - PubMed
    1. Sarantuya J, Nishi J, Wakimoto N, Erdene S, Nataro JP, Sheikh J, et al. Typical enteroaggregative Escherichia coli is the most prevalent pathotype among E. coli strains causing diarrhea in Mongolian children. J Clin Microbiol. 2004;42(1):133–9. - PMC - PubMed
    1. Franca FL, Wells TJ, Browning DF, Nogueira RT, Sarges FS, Pereira AC, et al. Genotypic and phenotypic characterisation of enteroaggregative Escherichia coli from children in Rio de Janeiro, Brazil. PLoS One. 2013;8(7):e22295. doi: 10.1371/journal.pone.0069971. - DOI - PMC - PubMed
    1. Nataro JP, Kaper JB, Robins-Browne R, Prado V, Vial P, Levine MM. Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr Infect Dis J. 1987;6(9):829–31. - PubMed

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