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. 2016 Apr;64(2):476-82.
doi: 10.1007/s12026-015-8706-5.

Highlighting the problematic reliance on CD18 for diagnosing leukocyte adhesion deficiency type 1

Sarina Levy-Mendelovich  1   2   3 , Erez Rechavi  1   2   3 , Omar Abuzaitoun  4 , Helly Vernitsky  5 , Amos J Simon  1   2   3   5 , Atar Lev  1   2   3 , Raz Somech  6   7   8
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Highlighting the problematic reliance on CD18 for diagnosing leukocyte adhesion deficiency type 1

Sarina Levy-Mendelovich et al. Immunol Res. 2016 Apr.

Abstract

Leukocyte adhesion deficiency type 1 (LAD-1) is an autosomal recessive primary immunodeficiency, hallmarked by defective polymorphonuclear transmigration. It is caused by mutations in the gene encoding CD18, which interfere with the CD18/CD11 heterodimerization and expression on leukocyte cell surface. LAD-1 diagnosis rests primarily on the measurement of CD18 expression. However, CD18 measurement entails its pitfalls. Here we present a cohort of ten LAD patients and a review of the relevant literature illustrating the difficulties in sole reliance on CD18 measurement for initial diagnosis. These include normal range expression in some mutations, great variability between patients with the same mutation and subjective interpretation of results. We think there is a need for additional markers as part of the initial LAD diagnostic algorithm. We suggest CD11a expression, which was near absent in all patients in our cohort. The dual use of CD18 and CD11a can increase testing sensitivity and prevent delayed diagnosis of LAD-1.

Keywords: CD11a; CD18; FACS; Leukocyte adhesion deficiency.

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References

    1. Exp Hematol. 2002 Mar;30(3):252-61 - PubMed
    1. Curr Opin Hematol. 2013 Jan;20(1):16-25 - PubMed
    1. Blood. 1990 Mar 1;75(5):1037-50 - PubMed
    1. J Clin Immunol. 2010 Sep;30(5):756-60 - PubMed
    1. Biol Blood Marrow Transplant. 2011 Aug;17(8):1245-9 - PubMed

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