Role of Magnesium Transporter Subtype 1 (MagT1) in the Osteogenic Differentiation of Rat Bone Marrow Stem Cells

doi:10.1007/s12011-015-0459-4

. 2016 May;171(1):131-7.

doi: 10.1007/s12011-015-0459-4. Epub 2015 Sep 11.

Role of Magnesium Transporter Subtype 1 (MagT1) in the Osteogenic Differentiation of Rat Bone Marrow Stem Cells

Jianmao Zheng^{1

2}, Xueli Mao^{1

2}, Junqi Ling^{3

4}, Chanchan Chen^{1

2}, Wen Zhang^{1

2}

Affiliations

¹ Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Sun Yat-sen University, Guangzhou, 510055, Guangdong, China.
² Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.
³ Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Sun Yat-sen University, Guangzhou, 510055, Guangdong, China. lingjq@mail.sysu.edu.cn.
⁴ Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China. lingjq@mail.sysu.edu.cn.

PMID: 26358767
DOI: 10.1007/s12011-015-0459-4

Role of Magnesium Transporter Subtype 1 (MagT1) in the Osteogenic Differentiation of Rat Bone Marrow Stem Cells

Jianmao Zheng et al. Biol Trace Elem Res. 2016 May.

. 2016 May;171(1):131-7.

doi: 10.1007/s12011-015-0459-4. Epub 2015 Sep 11.

Authors

Jianmao Zheng^{1

2}, Xueli Mao^{1

2}, Junqi Ling^{3

4}, Chanchan Chen^{1

2}, Wen Zhang^{1

2}

Affiliations

¹ Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Sun Yat-sen University, Guangzhou, 510055, Guangdong, China.
² Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China.
³ Department of Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Sun Yat-sen University, Guangzhou, 510055, Guangdong, China. lingjq@mail.sysu.edu.cn.
⁴ Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China. lingjq@mail.sysu.edu.cn.

PMID: 26358767
DOI: 10.1007/s12011-015-0459-4

Abstract

In the present study, we investigated the role of magnesium transporter subtype 1 (MagT1), a selective Mg transporter protein, in the osteogenic differentiation of rat bone marrow stem cells (rBMSCs). Osteogenic differentiation was monitored by the expressions of alkaline phosphatase (ALP), osteocalcin (OCN), collagen-1 (COL-1) and runt-related transcription factor 2 (RUNX2), and extracellular matrix mineralization of rBMSCs. The expression of MagT1 increased with osteogenic differentiation of rBMSCs, suggesting the importance of intracellular Mg homeostasis to cell differentiation. Alteration of intracellular Mg homeostasis by culture condition with low extracellular Mg significantly reduced the osteogenic differentiation markers ALP, OCN, COL-1, and RUNX2 gene expressions. MagT1 knockdown during the differentiation period also reduced osteogenic differentiation and the extent of matrix mineralization of rBMSCs. In conclusion, our results indicate that Mg and MagT1 play an important role in osteogenic differentiation of rBMSCs and may be involved in the bone regeneration.

Keywords: Bone marrow stem cells; Magnesium; Magnesium transporter subtype 1; Osteogenic differentiation.

PubMed Disclaimer

Cited by

A Review of the Action of Magnesium on Several Processes Involved in the Modulation of Hematopoiesis.
Lima FDS, Fock RA. Lima FDS, et al. Int J Mol Sci. 2020 Sep 25;21(19):7084. doi: 10.3390/ijms21197084. Int J Mol Sci. 2020. PMID: 32992944 Free PMC article. Review.
Magnesium Ammonium Phosphate Composite Cell-Laden Hydrogel Promotes Osteogenesis and Angiogenesis In Vitro.
Liu C, Yang G, Zhou M, Zhang X, Wu X, Wu P, Gu X, Jiang X. Liu C, et al. ACS Omega. 2021 Apr 2;6(14):9449-9459. doi: 10.1021/acsomega.0c06083. eCollection 2021 Apr 13. ACS Omega. 2021. PMID: 33869925 Free PMC article.
Osteogenic ability using porous hydroxyapatite scaffold-based delivery of human placenta-derived mesenchymal stem cells.
Ren X, Wang Q, Liu C, Zhao Q, Zheng J, Tian K, Xu H, Mu Y. Ren X, et al. Exp Ther Med. 2021 Oct;22(4):1091. doi: 10.3892/etm.2021.10525. Epub 2021 Aug 2. Exp Ther Med. 2021. PMID: 34504545 Free PMC article.
Superior biocompatibility and comparable osteoinductive properties: Sodium-reduced fluoride-containing bioactive glass belonging to the CaO-MgO-SiO₂ system as a promising alternative to 45S5 bioactive glass.
Schmitz SI, Widholz B, Essers C, Becker M, Tulyaganov DU, Moghaddam A, Gonzalo de Juan I, Westhauser F. Schmitz SI, et al. Bioact Mater. 2020 Jan 13;5(1):55-65. doi: 10.1016/j.bioactmat.2019.12.005. eCollection 2020 Mar. Bioact Mater. 2020. PMID: 31956736 Free PMC article.
N-linked glycosylation and homeostasis of the endoplasmic reticulum.
Cherepanova N, Shrimal S, Gilmore R. Cherepanova N, et al. Curr Opin Cell Biol. 2016 Aug;41:57-65. doi: 10.1016/j.ceb.2016.03.021. Epub 2016 Apr 14. Curr Opin Cell Biol. 2016. PMID: 27085638 Free PMC article. Review.

See all "Cited by" articles

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
- Springer
Medical
- MedlinePlus Health Information
Molecular Biology Databases
- GlyGen glycoinformatics resource

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Role of Magnesium Transporter Subtype 1 (MagT1) in the Osteogenic Differentiation of Rat Bone Marrow Stem Cells

Affiliations

Role of Magnesium Transporter Subtype 1 (MagT1) in the Osteogenic Differentiation of Rat Bone Marrow Stem Cells

Authors

Affiliations

Abstract

Similar articles

Cited by

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Molecular Biology Databases

Abstract

Similar articles

Cited by

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Medical

Molecular Biology Databases